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Literature summary for 6.2.1.12 extracted from

  • Yuan, Y.; Yu, S.; Yu, J.; Zhan, Z.; Li, M.; Liu, G.; Wang, X.; Huang, L.
    Predicting the function of 4-coumarate:CoA Ligase (LJ4CL1) in Lonicera japonica (2014), Int. J. Mol. Sci., 15, 2386-2399.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, genotyping and phylogenetic analysis Lonicera japonica

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Lonicera japonica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-coumarate + CoA Lonicera japonica
-
AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + ferulate + CoA Lonicera japonica
-
AMP + diphosphate + feruloyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Lonicera japonica
-
var. chinensis
-
Lonicera japonica L7Z3F8
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Lonicera japonica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-coumarate + CoA
-
Lonicera japonica AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + ferulate + CoA
-
Lonicera japonica AMP + diphosphate + feruloyl-CoA
-
?

Synonyms

Synonyms Comment Organism
4-coumarate:CoA ligase
-
Lonicera japonica
LJ4CL1
-
Lonicera japonica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Lonicera japonica

Cofactor

Cofactor Comment Organism Structure
ATP
-
Lonicera japonica

General Information

General Information Comment Organism
evolution the enzyme belongs to the proteins containing the GEICIRG motif, phylogeny based on AMP-dependent synthetase/ligase domain sequences, overview Lonicera japonica
metabolism 4-coumarate:CoA ligases are a group of essential enzymes involved in the phenylpropanoid-derived compound pathway, which converts hydroxylated cinnamic acids into their corresponding thioesters. The PDC pathway as well as its branch pathways generates various classes of secondary compounds, including lignin, flavones, flavonols, anthocyanins, isoflavonoids, and furanocoumarins Lonicera japonica
additional information putative substrate binding residues are I196, Y197, G317, G343, P349, V350, and L351 Lonicera japonica