Any feedback?
Literature summary for 6.2.1.12 extracted from
- Unusually divergent 4-coumarate:CoA-ligases from Ruta graveolens L (2008), Plant Mol. Biol., 67, 335-346.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae | Ruta graveolens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0031 | - |
4-coumarate | isozyme 4CL2, at pH 7.8 and 32°C | Ruta graveolens |  |
| 0.0055 | - |
4-coumarate | isozyme 4CL1, pH 7.8, 32°C | Ruta graveolens | |
| 0.037 | - |
caffeate | isozyme 4CL2, at pH 7.8 and 32°C | Ruta graveolens | |
| 0.037 | - |
cinnamate | isozyme 4CL2, at pH 7.8 and 32°C | Ruta graveolens | |
| 0.0484 | - |
caffeate | isozyme 4CL1, pH 7.8, 32°C | Ruta graveolens | |
| 0.059 | - |
cinnamate | isozyme 4CL1, pH 7.8, 32°C | Ruta graveolens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62380 | - |
calculated from amino acid sequence, isozyme 4CL1 | Ruta graveolens |
| 62384 | - |
x * 62384, calculated from amino acid sequence, isozyme 4CL1 | Ruta graveolens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ruta graveolens | B3TJI4 | isozyme 4CL1 | - |
| Ruta graveolens | B3TJI5 | isozyme 4CL2 | - |
| Ruta graveolens R-20 | B3TJI4 | isozyme 4CL1 | - |
| Ruta graveolens R-20 | B3TJI5 | isozyme 4CL2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sephadex G-25 gel filtration | Ruta graveolens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Ruta graveolens | - |
| flower | isozyme 4CL1 is mostly expressed in the flower | Ruta graveolens | - |
| leaf | - |
Ruta graveolens | - |
| root | negligible expression in the root | Ruta graveolens | - |
| shoot | - |
Ruta graveolens | - |
| shoot | isozyme 4CL2 is expressed mostly in the shoot | Ruta graveolens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4-coumarate + CoA | 100% activity | Ruta graveolens | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
| ATP + 4-coumarate + CoA | 100% activity | Ruta graveolens R-20 | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
| ATP + caffeate + CoA | 48% activity compared to 4-coumarate | Ruta graveolens | AMP + diphosphate + caffeoyl-CoA | - |
? | |
| ATP + caffeate + CoA | 86% activity compared to 4-coumarate | Ruta graveolens | AMP + diphosphate + caffeoyl-CoA | - |
? | |
| ATP + caffeate + CoA | 86% activity compared to 4-coumarate | Ruta graveolens R-20 | AMP + diphosphate + caffeoyl-CoA | - |
? | |
| ATP + caffeate + CoA | 48% activity compared to 4-coumarate | Ruta graveolens R-20 | AMP + diphosphate + caffeoyl-CoA | - |
? | |
| ATP + cinnamate + CoA | 5.0% activity compared to 4-coumarate | Ruta graveolens | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
| ATP + cinnamate + CoA | 54% activity compared to 4-coumarate | Ruta graveolens | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
| ATP + cinnamate + CoA | 54% activity compared to 4-coumarate | Ruta graveolens R-20 | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
| ATP + cinnamate + CoA | 5.0% activity compared to 4-coumarate | Ruta graveolens R-20 | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
| ATP + ferulate + CoA | 2.5% activity compared to 4-coumarate | Ruta graveolens | AMP + diphosphate + feruloyl-CoA | - |
? | |
| ATP + ferulate + CoA | 50.5% activity compared to 4-coumarate | Ruta graveolens | AMP + diphosphate + feruloyl-CoA | - |
? | |
| ATP + ferulate + CoA | 2.5% activity compared to 4-coumarate | Ruta graveolens R-20 | AMP + diphosphate + feruloyl-CoA | - |
? | |
| ATP + ferulate + CoA | 50.5% activity compared to 4-coumarate | Ruta graveolens R-20 | AMP + diphosphate + feruloyl-CoA | - |
? | |
| additional information | the enzyme does not activate sinapate, 2,4-dihydroxybenzoic acid, 4-hydroxybenzoic acid, acetylsalicylic acid, anthranilic acid, and (N-methyl)anthranilic acid | Ruta graveolens | ? | - |
? | |
| additional information | the enzyme does not activate sinapate, 2,4-dihydroxybenzoic acid, 4-hydroxybenzoic acid, acetylsalicylic acid, anthranilic acid, and (N-methyl)anthranilic acid | Ruta graveolens R-20 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62384, calculated from amino acid sequence, isozyme 4CL1 | Ruta graveolens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4-coumarate:CoA-ligase | - |
Ruta graveolens |
| 4CL | - |
Ruta graveolens |
| 4CL1 | isozyme | Ruta graveolens |
| 4CL2 | isozyme | Ruta graveolens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Ruta graveolens | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Ruta graveolens | calculated from amino acid sequence, isozyme 4CL1 | - |
5.8 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
