Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for developing effective plant growth inhibitors, overview | Nicotiana tabacum |
Cloned (Comment) | Organism |
---|---|
gene 4CL, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM105 | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | in vitro inhibitor screening using existing herbicides, screening assay optimization, overview | Nicotiana tabacum | |
propanil | a herbicide, 71.8% inhibition at 0.1 mM, uncompetitive inhibition towards 4-coumaric acid | Nicotiana tabacum | |
swep | a herbicide, 97.1% inhibition at 0.1 mM, uncompetitive inhibition towards 4-coumaric acid | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics in presence of inhibitors | Nicotiana tabacum | |
0.246 | - |
4-coumaric acid | - |
Nicotiana tabacum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | Nicotiana tabacum | the enzyme is involved in the phenylpropanoid pathway | AMP + diphosphate + 4-coumaroyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
gene 4CL | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain JM105 by a multistep process involving anion exchange chromatography and gel filtration | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | - |
Nicotiana tabacum | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | the enzyme is involved in the phenylpropanoid pathway | Nicotiana tabacum | AMP + diphosphate + 4-coumaroyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-coumarate:CoA ligase | - |
Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Nicotiana tabacum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.006 | - |
- |
Nicotiana tabacum | swep | |
0.0396 | - |
- |
Nicotiana tabacum | propanil |