Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for developing effective plant growth inhibitors, overview | Nicotiana tabacum |
Cloned (Comment) | Organism |
---|---|
gene 4CL, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM105 | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alachlor | slight inhibition | Nicotiana tabacum | |
bentazone | slight inhibition | Nicotiana tabacum | |
butachlor | - |
Nicotiana tabacum | |
chlornitrofen | - |
Nicotiana tabacum | |
dimepiperate | - |
Nicotiana tabacum | |
fenoxaprop-ethyl | - |
Nicotiana tabacum | |
linuron | slight inhibition | Nicotiana tabacum | |
mefenacet | slight inhibition | Nicotiana tabacum | |
molinate | slight inhibition | Nicotiana tabacum | |
additional information | development and optimization of a high-throughput miniaturized screening system of plant growth inhibitors targeting 4CL in the phenylpropanoid pathway, in vitro inhibitor screening using 28 existing herbicides, overview, no inhibition by pendimethalin, trifluralin, alloxydim-sodium, bispyribac-sodium, dimethenamid, metolachlor, pyrazolate, bensulfuron-methyl, and imazosulfuron | Nicotiana tabacum | |
nitrofen | slight inhibition | Nicotiana tabacum | |
Pentachlorophenol | slight inhibition | Nicotiana tabacum | |
pretilachlor | slight inhibition | Nicotiana tabacum | |
propanil | 72% inhibition at 0.1 mM | Nicotiana tabacum | |
pyrazosulfuron-ethyl | slight inhibition | Nicotiana tabacum | |
simazine | slight inhibition | Nicotiana tabacum | |
simetryn | slight inhibition | Nicotiana tabacum | |
swep | 98% inhibition at 0.1 mM | Nicotiana tabacum | |
thenylchlor | - |
Nicotiana tabacum | |
thiobencarb | slight inhibition | Nicotiana tabacum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | Nicotiana tabacum | the enzyme is involved in the phenylpropanoid pathway | AMP + diphosphate + 4-coumaroyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
gene 4CL | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 6.3fold from Escherichia coli strain JM105 by a multistep process involving anion exchange chromatography and gel filtration | Nicotiana tabacum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.624 | - |
purified recombinnat enzyme, substrate 4-coumaric acid | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | the enzyme is involved in the phenylpropanoid pathway | Nicotiana tabacum | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | 4-hydroxycinnamic acid, 98%, predominantly trans-isomer | Nicotiana tabacum | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + caffeic acid + CoA | 3,4-dihydroxycinnamic acid, 98% | Nicotiana tabacum | AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + caffeic acid + CoA | 3,4-dihydroxycinnamic acid, 98%, predominantly trans-isomer | Nicotiana tabacum | AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + cinnamic acid + CoA | 3-phenyl-2-propenoic acid, 98% | Nicotiana tabacum | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
ATP + ferulic acid + CoA | 4-hydroxy-3-methoxycinnamic acid, 99% | Nicotiana tabacum | AMP + diphosphate + 4-feruloyl-CoA | - |
? | |
ATP + sinapic acid + CoA | 3,5-dimethoxy-4-hydroxycinnamic acid, 98%, predominantly trans isomer | Nicotiana tabacum | AMP + diphosphate + 4-sinapoyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-coumarate:CoA ligase | - |
Nicotiana tabacum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Nicotiana tabacum |