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Literature summary for 6.2.1.12 extracted from

  • Yun, M.S.; Chen, W.; Deng, F.; Kiyokawa, T.; Mametsuka, K.; Yogo, Y.
    An in vitro screening assay to discover novel inhibitors of 4-coumarate:CoA ligase (2006), Pest Manag. Sci., 62, 1065-1071.
    View publication on PubMed

Application

Application Comment Organism
drug development the enzyme is a target for developing effective plant growth inhibitors, overview Nicotiana tabacum

Cloned(Commentary)

Cloned (Comment) Organism
gene 4CL, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM105 Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
alachlor slight inhibition Nicotiana tabacum
bentazone slight inhibition Nicotiana tabacum
butachlor
-
Nicotiana tabacum
chlornitrofen
-
Nicotiana tabacum
dimepiperate
-
Nicotiana tabacum
fenoxaprop-ethyl
-
Nicotiana tabacum
linuron slight inhibition Nicotiana tabacum
mefenacet slight inhibition Nicotiana tabacum
molinate slight inhibition Nicotiana tabacum
additional information development and optimization of a high-throughput miniaturized screening system of plant growth inhibitors targeting 4CL in the phenylpropanoid pathway, in vitro inhibitor screening using 28 existing herbicides, overview, no inhibition by pendimethalin, trifluralin, alloxydim-sodium, bispyribac-sodium, dimethenamid, metolachlor, pyrazolate, bensulfuron-methyl, and imazosulfuron Nicotiana tabacum
nitrofen slight inhibition Nicotiana tabacum
Pentachlorophenol slight inhibition Nicotiana tabacum
pretilachlor slight inhibition Nicotiana tabacum
propanil 72% inhibition at 0.1 mM Nicotiana tabacum
pyrazosulfuron-ethyl slight inhibition Nicotiana tabacum
simazine slight inhibition Nicotiana tabacum
simetryn slight inhibition Nicotiana tabacum
swep 98% inhibition at 0.1 mM Nicotiana tabacum
thenylchlor
-
Nicotiana tabacum
thiobencarb slight inhibition Nicotiana tabacum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-coumarate + CoA Nicotiana tabacum the enzyme is involved in the phenylpropanoid pathway AMP + diphosphate + 4-coumaroyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
gene 4CL
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 6.3fold from Escherichia coli strain JM105 by a multistep process involving anion exchange chromatography and gel filtration Nicotiana tabacum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.624
-
purified recombinnat enzyme, substrate 4-coumaric acid Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-coumarate + CoA the enzyme is involved in the phenylpropanoid pathway Nicotiana tabacum AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + 4-coumarate + CoA 4-hydroxycinnamic acid, 98%, predominantly trans-isomer Nicotiana tabacum AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + caffeic acid + CoA 3,4-dihydroxycinnamic acid, 98% Nicotiana tabacum AMP + diphosphate + caffeoyl-CoA
-
?
ATP + caffeic acid + CoA 3,4-dihydroxycinnamic acid, 98%, predominantly trans-isomer Nicotiana tabacum AMP + diphosphate + caffeoyl-CoA
-
?
ATP + cinnamic acid + CoA 3-phenyl-2-propenoic acid, 98% Nicotiana tabacum AMP + diphosphate + cinnamoyl-CoA
-
?
ATP + ferulic acid + CoA 4-hydroxy-3-methoxycinnamic acid, 99% Nicotiana tabacum AMP + diphosphate + 4-feruloyl-CoA
-
?
ATP + sinapic acid + CoA 3,5-dimethoxy-4-hydroxycinnamic acid, 98%, predominantly trans isomer Nicotiana tabacum AMP + diphosphate + 4-sinapoyl-CoA
-
?

Synonyms

Synonyms Comment Organism
4-coumarate:CoA ligase
-
Nicotiana tabacum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
ATP
-
Nicotiana tabacum