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Literature summary for 6.2.1.12 extracted from

  • Pietrowska-Borek, M.; Stuible, H.P.; Kombrink, E.; Guranowski, A.
    4-Coumarate:coenzyme A ligase has the catalytic capacity to synthesize and reuse various (di)adenosine polyphosphates (2003), Plant Physiol., 131, 1401-1410.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
M293P/K320L strongly affects Km for all substrates, capable in using ferulate, capable in adenosine 5'-tetraphosphate formation at 10fold higher rate than wild type enzyme Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
ATP wild-type enzyme, pH 7.0, 30°C Arabidopsis thaliana
0.024
-
caffeate wild-type enzyme, pH 7.0, 30°C Arabidopsis thaliana
0.027
-
4-coumarate M293P/K320L mutant enzyme, pH 7.0, 30°C Arabidopsis thaliana
0.046
-
ferulate M293P/K320L mutant enzyme, pH 7.0, 30°C Arabidopsis thaliana
0.048
-
caffeate M293P/K320L mutant enzyme, pH 7.0, 30°C Arabidopsis thaliana
0.064
-
adenosine 5'-tetraphosphate reverse reaction, M293P/K320L mutant enzyme, pH 7.0, 30°C Arabidopsis thaliana
0.233
-
4-coumarate wild-type enzyme, pH 7.0, 30°C Arabidopsis thaliana
0.292
-
cinnamate M293P/K320L mutant enzyme, pH 7.0, 30°C Arabidopsis thaliana
6.35
-
cinnamate wild-type enzyme, pH 7.0, 30°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially substitute for Mg2+ Arabidopsis thaliana
Mg2+ required Arabidopsis thaliana
Mn2+ can partially substitute for Mg2+ Arabidopsis thaliana
additional information no effect with Ca2+ Arabidopsis thaliana
Ni2+ can partially substitute for Mg2+ Arabidopsis thaliana
Zn2+ can partially substitute for Mg2+, very low activity Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-coumarate + CoA Arabidopsis thaliana plant phenylpropanoid metabolism, 4CL2 involved in coenzyme A ester formation and cocatalytic AMP-donor in (di)adenosine polyphosphate synthesis AMP + diphosphate + 4-coumaroyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
isoform 4CL2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumarate + MgATP2- + tetrapolyphosphate CoA omitted from the incubation mixture Arabidopsis thaliana adenosine 5'-pentaphosphate + ?
-
r
4-coumarate + MgATP2- + tripolyphosphate CoA omitted from the incubation mixture Arabidopsis thaliana adenosine 5'-tetraphosphate + ?
-
r
ATP + 4-coumarate + CoA
-
Arabidopsis thaliana AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + 4-coumarate + CoA plant phenylpropanoid metabolism, 4CL2 involved in coenzyme A ester formation and cocatalytic AMP-donor in (di)adenosine polyphosphate synthesis Arabidopsis thaliana AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + caffeate + CoA
-
Arabidopsis thaliana AMP + diphosphate + caffeoyl-CoA
-
?
ATP + cinnamate + CoA
-
Arabidopsis thaliana AMP + diphosphate + cinnamoyl-CoA
-
?
ATP + ferulate + CoA M293P/K320L mutant enzyme, no substrate for wild type enzyme Arabidopsis thaliana AMP + diphosphate + feruloyl-CoA
-
?
ATP + sinapate + CoA
-
Arabidopsis thaliana AMP + diphosphate + sinapoyl-CoA
-
?
MgATP2- no additional substrate, CoA omitted from the incubation mixture Arabidopsis thaliana diadenosine 5',5'',P1,P4-tetraphosphate + ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8 adenosine 5' tetraphosphate formation Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9 half maximal activity, adenosine 5' tetraphosphate foormation Arabidopsis thaliana