Cloned (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
M293P/K320L | strongly affects Km for all substrates, capable in using ferulate, capable in adenosine 5'-tetraphosphate formation at 10fold higher rate than wild type enzyme | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
ATP | wild-type enzyme, pH 7.0, 30°C | Arabidopsis thaliana | |
0.024 | - |
caffeate | wild-type enzyme, pH 7.0, 30°C | Arabidopsis thaliana | |
0.027 | - |
4-coumarate | M293P/K320L mutant enzyme, pH 7.0, 30°C | Arabidopsis thaliana | |
0.046 | - |
ferulate | M293P/K320L mutant enzyme, pH 7.0, 30°C | Arabidopsis thaliana | |
0.048 | - |
caffeate | M293P/K320L mutant enzyme, pH 7.0, 30°C | Arabidopsis thaliana | |
0.064 | - |
adenosine 5'-tetraphosphate | reverse reaction, M293P/K320L mutant enzyme, pH 7.0, 30°C | Arabidopsis thaliana | |
0.233 | - |
4-coumarate | wild-type enzyme, pH 7.0, 30°C | Arabidopsis thaliana | |
0.292 | - |
cinnamate | M293P/K320L mutant enzyme, pH 7.0, 30°C | Arabidopsis thaliana | |
6.35 | - |
cinnamate | wild-type enzyme, pH 7.0, 30°C | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially substitute for Mg2+ | Arabidopsis thaliana | |
Mg2+ | required | Arabidopsis thaliana | |
Mn2+ | can partially substitute for Mg2+ | Arabidopsis thaliana | |
additional information | no effect with Ca2+ | Arabidopsis thaliana | |
Ni2+ | can partially substitute for Mg2+ | Arabidopsis thaliana | |
Zn2+ | can partially substitute for Mg2+, very low activity | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | Arabidopsis thaliana | plant phenylpropanoid metabolism, 4CL2 involved in coenzyme A ester formation and cocatalytic AMP-donor in (di)adenosine polyphosphate synthesis | AMP + diphosphate + 4-coumaroyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
isoform 4CL2 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumarate + MgATP2- + tetrapolyphosphate | CoA omitted from the incubation mixture | Arabidopsis thaliana | adenosine 5'-pentaphosphate + ? | - |
r | |
4-coumarate + MgATP2- + tripolyphosphate | CoA omitted from the incubation mixture | Arabidopsis thaliana | adenosine 5'-tetraphosphate + ? | - |
r | |
ATP + 4-coumarate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | plant phenylpropanoid metabolism, 4CL2 involved in coenzyme A ester formation and cocatalytic AMP-donor in (di)adenosine polyphosphate synthesis | Arabidopsis thaliana | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + caffeate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + cinnamate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
ATP + ferulate + CoA | M293P/K320L mutant enzyme, no substrate for wild type enzyme | Arabidopsis thaliana | AMP + diphosphate + feruloyl-CoA | - |
? | |
ATP + sinapate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + sinapoyl-CoA | - |
? | |
MgATP2- | no additional substrate, CoA omitted from the incubation mixture | Arabidopsis thaliana | diadenosine 5',5'',P1,P4-tetraphosphate + ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | adenosine 5' tetraphosphate formation | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9 | half maximal activity, adenosine 5' tetraphosphate foormation | Arabidopsis thaliana |