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Literature summary for 6.2.1.12 extracted from

  • Harding, S.A.; Leshkevich, J.; Chiang, V.L.; Tsai, C.J.
    Differential substrate inhibition couples kinetically distinct 4-coumarate: coenzyme A ligases with spatially distinct metabolic roles in quaking aspen (2002), Plant Physiol., 128, 428-438.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as His-tag fusion protein Pinus taeda
expressed in Escherichia coli as His-tag fusion protein Populus tremuloides

Inhibitors

Inhibitors Comment Organism Structure
4-coumarate strong competitive inhibitor for the other substrates Populus tremuloides
caffeate strong competitive inhibitor for the other substrates Populus tremuloides
ferulate strong competitive inhibitor for the other substrates Populus tremuloides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0036
-
 4-coumarate 4CL2, pH 7.5, 30°C Populus tremuloides
0.035
-
 caffeate 4CL1, pH 7.5, 30°C Populus tremuloides
0.042
-
 caffeate 4CL2, pH 7.5, 30°C Populus tremuloides
0.056
-
 4-coumarate 4CL1, pH 7.5, 30°C Populus tremuloides
0.112
-
 ferulate 4CL1, pH 7.5, 30°C Populus tremuloides
0.139
-
 ferulate 4CL2, pH 7.5, 30°C Populus tremuloides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-coumarate + CoA Pinus taeda plant phenylpropanoid metabolism AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 4-coumarate + CoA Populus tremuloides plant phenylpropanoid metabolism AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Pinus taeda
-
 no isoforms detected, one protein with broad substrate specificity
-
Populus tremuloides
-
 two isoforms, 4CL1 and 4CL2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Pinus taeda
recombinant protein using His-tag Populus tremuloides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-coumarate + CoA
-
 Pinus taeda AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 4-coumarate + CoA
-
 Populus tremuloides AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 4-coumarate + CoA plant phenylpropanoid metabolism Pinus taeda AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 4-coumarate + CoA plant phenylpropanoid metabolism Populus tremuloides AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + caffeate + CoA
-
 Populus tremuloides AMP + diphosphate + caffeoyl-CoA
-
 ?
ATP + caffeate + CoA same activity as with 4-coumarate Pinus taeda AMP + diphosphate + caffeoyl-CoA
-
 ?
ATP + ferulate + CoA only 4CL1, no substrate for 4CL2 Populus tremuloides AMP + diphosphate + feruloyl-CoA
-
 ?
ATP + ferulate + CoA same activity as with 4-coumarate Pinus taeda AMP + diphosphate + feruloyl-CoA
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.004
-
 caffeate 4CL1 with 4-coumarate as substrate, pH 7.5, 30°C Populus tremuloides
0.012
-
 caffeate 4CL2 with 4-coumarate as substrate, pH 7.5, 30°C Populus tremuloides
0.1
-
 ferulate 4CL1 with 4-coumarate as substrate, pH 7.5, 30°C Populus tremuloides
0.3
-
 ferulate 4CL2 with 4-coumarate as substrate, pH 7.5, 30°C Populus tremuloides