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Literature summary for 6.2.1.12 extracted from

  • Ehlting, J.; Shin, J.J.; Douglas, C.J.
    Identification of 4-coumarate:coenzyme A ligase (4CL) substrate recognition domains (2001), Plant J., 27, 455-465.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15 as His-tag fusion protein Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of chimeric proteins consisting of fragments of isoforms 4CL1 and 4CL2 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
4-coumarate 4CL1, pH 7.8, room temperature Arabidopsis thaliana
0.057
-
ferulate 4CL1, pH 7.8, room temperature Arabidopsis thaliana
0.22
-
4-coumarate 4CL2, pH 7.8, room temperature Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-coumarate + CoA Arabidopsis thaliana plant phenylpropanoid metabolism AMP + diphosphate + 4-coumaroyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
isoforms 4CL1 and 4CL2
-

Purification (Commentary)

Purification (Comment) Organism
using His-tag Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-coumarate + CoA
-
Arabidopsis thaliana AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + 4-coumarate + CoA plant phenylpropanoid metabolism Arabidopsis thaliana AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + ferulate + CoA only 4CL1, no substrate for 4CL2 Arabidopsis thaliana AMP + diphosphate + feruloyl-CoA
-
?