Literature summary for 6.2.1.12 extracted from

Ehlting, J.; Buttner, D.; Wang, Q.; Douglas, C.J.; Somssich, I.E.; Kombrink, E.
Three 4-coumarate:coenzyme A ligases in Arabidopsis thaliana represent two evolutionarily divergent classes in angiosperms (1999), Plant J., 19, 9-20.

Search for more literature for 6.2.1.12

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli as His-tag fusion protein and in native form	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.011	-	caffeate	room temperature	Arabidopsis thaliana
0.02	-	caffeate	room temperature	Arabidopsis thaliana
0.023	-	4-coumarate	room temperature	Arabidopsis thaliana
0.038	-	4-coumarate	room temperature	Arabidopsis thaliana
0.166	-	ferulate	room temperature	Arabidopsis thaliana
0.199	-	ferulate	room temperature	Arabidopsis thaliana
0.252	-	4-coumarate	room temperature	Arabidopsis thaliana
0.374	-	caffeate	room temperature	Arabidopsis thaliana
2.07	-	cinnamate	room temperature	Arabidopsis thaliana
6.32	-	cinnamate	room temperature	Arabidopsis thaliana
6.63	-	cinnamate	room temperature	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
66000	-	SDS-PAGE	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 4-coumarate + CoA	Arabidopsis thaliana	plant phenylpropanoid metabolism, class II enzymes part of flavonoid pathway, class I enzymes probably involved in lignin formation and phenolic compound other than flavonoids	AMP + diphosphate + 4-coumaroyl-CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q42524	isoform Cl1, genomic DNA; three isoforms with different substrate specificity and preference, isoforms clustered in class I and II with 4Cl1 and 4Cl2 belonging to class I and 4Cl3 belonging to class II	-
Arabidopsis thaliana	Q9S725	isoform 4Cl2, genomic DNA; three isoforms with different substrate specificity and preference, isoforms clustered in class I and II with 4Cl1 and 4Cl2 belonging to class I and 4Cl3 belonging to class II	-
Arabidopsis thaliana	Q9S725	isoform 4Cl3, genomic DNA; three isoforms with different substrate specificity and preference, isoforms clustered in class I and II with 4Cl1 and 4Cl2 belonging to class I and 4Cl3 belonging to class II	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 4-coumarate + CoA	-	Arabidopsis thaliana	AMP + diphosphate + 4-coumaroyl-CoA	-	?
ATP + 4-coumarate + CoA	plant phenylpropanoid metabolism, class II enzymes part of flavonoid pathway, class I enzymes probably involved in lignin formation and phenolic compound other than flavonoids	Arabidopsis thaliana	AMP + diphosphate + 4-coumaroyl-CoA	-	?
ATP + caffeate + CoA	-	Arabidopsis thaliana	AMP + diphosphate + caffeoyl-CoA	-	?
ATP + cinnamate + CoA	-	Arabidopsis thaliana	AMP + diphosphate + cinnamoyl-CoA	-	?
ATP + cinnamate + CoA	same Vmax like 4-coumarate	Arabidopsis thaliana	AMP + diphosphate + cinnamoyl-CoA	-	?
ATP + ferulate + CoA	-	Arabidopsis thaliana	AMP + diphosphate + feruloyl-CoA	-	?
additional information	inactive against sinapic acid	Arabidopsis thaliana	?	-	?
additional information	inactive against sinapic acid and ferulic acid	Arabidopsis thaliana	?	-	?

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)