Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as His-tag fusion protein | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
ferulate | - |
Glycine max | |
0.009 | - |
4-coumarate | - |
Glycine max | |
0.01 | - |
4-coumarate | - |
Glycine max | |
0.011 | - |
sinapate | - |
Glycine max | |
0.013 | - |
caffeate | - |
Glycine max | |
0.022 | - |
4-coumarate | - |
Glycine max | |
0.033 | - |
caffeate | - |
Glycine max | |
0.034 | - |
caffeate | - |
Glycine max | |
0.042 | - |
4-coumarate | - |
Glycine max | |
0.05 | - |
caffeate | - |
Glycine max | |
0.083 | - |
3,4-dimethoxycinnamate | - |
Glycine max | |
0.14 | - |
ferulate | - |
Glycine max | |
0.26 | - |
cinnamate | - |
Glycine max | |
1.1 | - |
cinnamate | - |
Glycine max | |
1.3 | - |
ferulate | - |
Glycine max | |
1.7 | - |
cinnamate | - |
Glycine max | |
3.1 | - |
ferulate | - |
Glycine max | |
4.4 | - |
cinnamate | - |
Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | Glycine max | plant phenylpropanoid metabolism | AMP + diphosphate + 4-coumaroyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | P31687 | 4CL4, cDNA; four isoforms 4CL1-4 | - |
Glycine max | Q8S564 | 4CL1, cDNA; four isoforms 4CL1-4 | - |
Glycine max | Q8S5C1 | 4CL2, cDNA; four isoforms 4CL1-4 | - |
Glycine max | Q8S5C2 | 4CL3, genomic DNA; four isoforms 4CL1-4 | - |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3,4-dimethoxycinnamate + CoA | - |
Glycine max | AMP + diphosphate + 3,4-dimethoxycinnamoyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | - |
Glycine max | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | plant phenylpropanoid metabolism | Glycine max | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + caffeate + CoA | - |
Glycine max | AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + cinnamate + CoA | - |
Glycine max | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
ATP + ferulate + CoA | - |
Glycine max | AMP + diphosphate + feruloyl-CoA | - |
? | |
ATP + sinapate + CoA | - |
Glycine max | AMP + diphosphate + sinapoyl-CoA | - |
? | |
additional information | no reaction with 3,4-dimethoxycinnamate and sinapate | Glycine max | ? | - |
? |