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Literature summary for 6.2.1.12 extracted from

  • Knobloch, K.H.; Hahlbrock, K.
    Isoenzymes of p-coumarate:CoA ligase from cell suspension cultures of Glycine max (1975), Eur. J. Biochem., 52, 311-320.
    View publication on PubMed
Search for more literature for 6.2.1.12

Inhibitors

Inhibitors Comment Organism Structure
4-coumarate above 0.1 mM Glycine max
CoASH above 0.1 mM Glycine max
Ionic strength activity of isoenzyme 1 decreases steadily with increasing ionic strength at least in the range from 0.1 to 0.8 M Tris-HCl, highest rate of isoenzyme 2 activity is obtained at concentrations of about 0.3 M Tris-HCl Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 CoASH isoenzyme 2 Glycine max
0.007
-
 CoASH isoenzyme 1 Glycine max
0.009
-
 ferulate isoenzyme 1 Glycine max
0.011
-
 sinapoate isoenzyme 1 Glycine max
0.012
-
 5-hydroxyferulate isoenzyme 1 Glycine max
0.014
-
 caffeate isoenzyme 2 Glycine max
0.017
-
 4-coumarate isoenzyme 2 Glycine max
0.03
-
 3-coumarate isoenzyme 2 Glycine max
0.032
-
 4-coumarate isoenzyme 1 Glycine max
0.04
-
 caffeate isoenzyme 1 Glycine max
0.065
-
 ATP isoenzyme 2 Glycine max
0.09
-
 2-coumarate isoenzyme 2 Glycine max
0.1
-
 isoferulate 3,4-dimethoxycinnamate, , isoenzyme 1 Glycine max
0.12
-
 5-hydroxyferulate isoenzyme 2 Glycine max
0.13
-
 ferulate isoenzyme 2 Glycine max
0.15
-
 isoferulate isoenzyme 2 Glycine max
0.17
-
 ATP 3-coumarate, , isoenzyme 1 Glycine max
0.18
-
 2-coumarate isoenzyme 1 Glycine max
0.23
-
 3,4,5-trimethoxycinnamate isoenzyme 1 Glycine max
0.38
-
 4-methoxycinnamate isoenzyme 1 Glycine max
1.3
-
 cinnamate isoenzyme 1 Glycine max
4.5
-
 cinnamate isoenzyme 2 Glycine max

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation required, Mg2+, Mn2+ and Co2+ are equally effective Glycine max
Mg2+ divalent cation required, Mg2+, Mn2+ and Co2+ are equally effective Glycine max
Mn2+ divalent cation required, Mg2+, Mn2+ and Co2+ are equally effective Glycine max

Organic Solvent Stability

Organic Solvent Comment Organism
Ethylene glycol 30% ethylene glycol stabilizes. Loss of activity after 7 days is reduced from 80% to about 25% Glycine max
Glycerol 30% glycerol stabilizes. Loss of activity after 7 days is reduced from 80% to about 25% Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
 var. Mandarin
-
Glycine max
-
 2 isoenzymes
-

Purification (Commentary)

Purification (Comment) Organism
partial Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Glycine max

Storage Stability

Storage Stability Organism
crude enzyme preparation is unstable in buffered aqueous solution at temperatures at or above 0°C Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-coumarate + CoA
-
 Glycine max AMP + diphosphate + 2-coumaroyl-CoA
-
 ?
ATP + 3,4,5-trimethoxycinnamate + CoA isoenzyme 1 is active, isoenzyme 2 not Glycine max AMP + diphosphate + 3,4,5-trimethoxycinnamoyl-CoA
-
 ?
ATP + 3,4-dimethoxycinnamate + CoA isoenzyme 1 is active, isoenzyme 2 not Glycine max AMP + diphosphate + 3,4-dimethoxycinnamoyl-CoA
-
 ?
ATP + 3-coumarate + CoA
-
 Glycine max AMP + diphosphate + 3-coumaroyl-CoA
-
 ?
ATP + 4-coumarate + CoA
-
 Glycine max AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 4-methoxycinnamate + CoA isoenzyme 1 is active, isoenzyme 2 not Glycine max AMP + diphosphate + 4-methoxycinnamoyl-CoA
-
 ?
ATP + 5-hydroxyferulate + CoA
-
 Glycine max AMP + diphosphate + 5-hydroxyferuloyl-CoA
-
 ?
ATP + caffeate + CoA
-
 Glycine max AMP + diphosphate + caffeoyl-CoA
-
 ?
ATP + cinnamate + CoA
-
 Glycine max AMP + diphosphate + cinnamoyl-CoA
-
 ?
ATP + ferulate + CoA
-
 Glycine max AMP + diphosphate + feruloyl-CoA
-
 ?
ATP + isoferulate + CoA
-
 Glycine max AMP + diphosphate + isoferuloyl-CoA
-
 ?
ATP + sinapoate + CoA isoenzyme 1 is active with sinapoate, isoenzyme 2 not Glycine max AMP + diphosphate + sinapoyl-CoA
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 formation of 4-coumaroyl-CoA, isoenzyme 2 Glycine max
8.3
-
 formation of 4-coumaroyl-CoA, isoenzyme 1 Glycine max