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Literature summary for 6.2.1.12 extracted from
- Isolation and properties of hydroxycinnamate:CoA ligase from lignifying tissues of Forsythia (1974), Eur. J. Biochem., 42, 453-459.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CoA | substrate inhibition | Forsythia suspensa |  |
| ferulate | strong substrate inhibition above 0.2 mM | Forsythia suspensa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0032 | - |
CoA | - |
Forsythia suspensa | |
| 0.02 | - |
ferulate | - |
Forsythia suspensa | |
| 0.036 | - |
ATP | - |
Forsythia suspensa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Forsythia suspensa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
gel filtration | Forsythia suspensa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Forsythia suspensa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Forsythia suspensa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| stem | young, lignifying | Forsythia suspensa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Forsythia suspensa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol | Forsythia suspensa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4-coumarate + CoA | - |
Forsythia suspensa | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
| ATP + ferulate + CoA | - |
Forsythia suspensa | AMP + diphosphate + feruloyl-CoA | - |
? | |
| CTP + ferulate + CoA | 7.7% of the activity relative to ATP | Forsythia suspensa | CMP + diphosphate + feruloyl-CoA | - |
? | |
| GTP + ferulate + CoA | 5.7% of the activity relative to ATP | Forsythia suspensa | GMP + diphosphate + feruloyl-CoA | - |
? | |
| ITP + ferulate + CoA | 20.2% of the activity relative to ATP | Forsythia suspensa | IMP + diphosphate + feruloyl-CoA | - |
? | |
| additional information | in ATP-diphosphate exchange reaction absolute specificity for cinnamate | Forsythia suspensa | ? | - |
? | |
| UTP + ferulate + CoA | 12.5% of the activity relative to GTP | Forsythia suspensa | UMP + diphosphate + feruloyl-CoA | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 47 | - |
rapid inactivation | Forsythia suspensa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
ATP-diphosphate exchange reaction | Forsythia suspensa |
| 7.3 | - |
formation of feruloyl-CoA | Forsythia suspensa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | stable | Forsythia suspensa |
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