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Literature summary for 6.2.1.1 extracted from

  • You, D.; Wang, M.M.; Ye, B.C.
    Acetyl-CoA synthetases of Saccharopolyspora erythraea are regulated by the nitrogen response regulator GlnR at both transcriptional and post-translational levels (2017), Mol. Microbiol., 103, 845-859 .
    View publication on PubMed
Search for more literature for 6.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharopolyspora erythraea

Protein Variants

Protein Variants Comment Organism
K615Q the mutant of isoform Acs3 retains 10% of wild type activity Saccharopolyspora erythraea
K615R the mutant of isoform Acs3 retains 10% of wild type activity Saccharopolyspora erythraea
K620Q the mutant of isoform Acs1 retains 10% of wild type activity Saccharopolyspora erythraea
K620R the mutant of isoform Acs1 retains 10% of wild type activity Saccharopolyspora erythraea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9
-
 acetate isoform Acs3, at pH 7.7 and 37°C Saccharopolyspora erythraea
3.3
-
 acetate isoform Acs2, at pH 7.7 and 37°C Saccharopolyspora erythraea
4.5
-
 acetate isoform Acs1, at pH 7.7 and 37°C Saccharopolyspora erythraea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate + CoA Saccharopolyspora erythraea
-
 AMP + diphosphate + acetyl-CoA
-
 ?
ATP + acetate + CoA Saccharopolyspora erythraea NRRL23338
-
 AMP + diphosphate + acetyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharopolyspora erythraea
-
-
-
Saccharopolyspora erythraea NRRL23338
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Saccharopolyspora erythraea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate + CoA
-
 Saccharopolyspora erythraea AMP + diphosphate + acetyl-CoA
-
 ?
ATP + acetate + CoA
-
 Saccharopolyspora erythraea NRRL23338 AMP + diphosphate + acetyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
acetyl CoA synthetase
-
 Saccharopolyspora erythraea
acsA1 isoform Saccharopolyspora erythraea
AcsA2 isoform Saccharopolyspora erythraea
AcsA3 isoform Saccharopolyspora erythraea
AMP-forming acetyl-CoA synthetase
-
 Saccharopolyspora erythraea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33.5
-
 acetate isoform Acs2, at pH 7.7 and 37°C Saccharopolyspora erythraea
52.7
-
 acetate isoform Acs3, at pH 7.7 and 37°C Saccharopolyspora erythraea
77.5
-
 acetate isoform Acs1, at pH 7.7 and 37°C Saccharopolyspora erythraea

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharopolyspora erythraea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10
-
 acetate isoform Acs2, at pH 7.7 and 37°C Saccharopolyspora erythraea
17
-
 acetate isoform Acs1, at pH 7.7 and 37°C Saccharopolyspora erythraea
18
-
 acetate isoform Acs3, at pH 7.7 and 37°C Saccharopolyspora erythraea