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Literature summary for 6.2.1.1 extracted from

  • Berger, S.; Welte, C.; Deppenmeier, U.
    Acetate activation in Methanosaeta thermophila: characterization of the key enzymes pyrophosphatase and acetyl-CoA synthetase (2012), Archaea, 2012, 315153.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Methanothrix thermoacetophila

Inhibitors

Inhibitors Comment Organism Structure
diphosphate addition of 0.25 mM diphosphate results in 50% reduction of the reaction rate Methanothrix thermoacetophila
additional information no inhibition by AMP, ADP, ATP or phosphate Methanothrix thermoacetophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0145
-
CoA pH 7.5, 55°C Methanothrix thermoacetophila
0.02
-
ATP pH 7.5, 55°C Methanothrix thermoacetophila
0.4
-
acetate pH 7.5, 55°C Methanothrix thermoacetophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
x * 75000, SDS-PAGE Methanothrix thermoacetophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate + CoA Methanothrix thermoacetophila activation of acetate in energy metabolism AMP + diphosphate + acetyl-CoA
-
?
ATP + acetate + CoA Methanothrix thermoacetophila DSM 6194 activation of acetate in energy metabolism AMP + diphosphate + acetyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Methanothrix thermoacetophila A0B8F1
-
-
Methanothrix thermoacetophila DSM 6194 A0B8F1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanothrix thermoacetophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28
-
pH 7.5, 55°C Methanothrix thermoacetophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate + CoA activation of acetate in energy metabolism Methanothrix thermoacetophila AMP + diphosphate + acetyl-CoA
-
?
ATP + acetate + CoA activity with propionate is 1% compared to the reactivity with acetate. Butyrate does not serve as a substrate Methanothrix thermoacetophila AMP + diphosphate + acetyl-CoA
-
?
ATP + acetate + CoA activation of acetate in energy metabolism Methanothrix thermoacetophila DSM 6194 AMP + diphosphate + acetyl-CoA
-
?
ATP + acetate + CoA activity with propionate is 1% compared to the reactivity with acetate. Butyrate does not serve as a substrate Methanothrix thermoacetophila DSM 6194 AMP + diphosphate + acetyl-CoA
-
?

Subunits

Subunits Comment Organism
? x * 75000, SDS-PAGE Methanothrix thermoacetophila

Synonyms

Synonyms Comment Organism
AMP-dependent ACS
-
Methanothrix thermoacetophila
AMP-forming acetyl-CoA synthetase
-
Methanothrix thermoacetophila
Mthe_1194 locus name Methanothrix thermoacetophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
assay at Methanothrix thermoacetophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
30 min, 15% loss of activity Methanothrix thermoacetophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Methanothrix thermoacetophila

General Information

General Information Comment Organism
physiological function activation of acetate in energy metabolism Methanothrix thermoacetophila