Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Methanothrix thermoacetophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | addition of 0.25 mM diphosphate results in 50% reduction of the reaction rate | Methanothrix thermoacetophila | |
additional information | no inhibition by AMP, ADP, ATP or phosphate | Methanothrix thermoacetophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0145 | - |
CoA | pH 7.5, 55°C | Methanothrix thermoacetophila | |
0.02 | - |
ATP | pH 7.5, 55°C | Methanothrix thermoacetophila | |
0.4 | - |
acetate | pH 7.5, 55°C | Methanothrix thermoacetophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
x * 75000, SDS-PAGE | Methanothrix thermoacetophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | Methanothrix thermoacetophila | activation of acetate in energy metabolism | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | Methanothrix thermoacetophila DSM 6194 | activation of acetate in energy metabolism | AMP + diphosphate + acetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothrix thermoacetophila | A0B8F1 | - |
- |
Methanothrix thermoacetophila DSM 6194 | A0B8F1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothrix thermoacetophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28 | - |
pH 7.5, 55°C | Methanothrix thermoacetophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | activation of acetate in energy metabolism | Methanothrix thermoacetophila | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | activity with propionate is 1% compared to the reactivity with acetate. Butyrate does not serve as a substrate | Methanothrix thermoacetophila | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | activation of acetate in energy metabolism | Methanothrix thermoacetophila DSM 6194 | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | activity with propionate is 1% compared to the reactivity with acetate. Butyrate does not serve as a substrate | Methanothrix thermoacetophila DSM 6194 | AMP + diphosphate + acetyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000, SDS-PAGE | Methanothrix thermoacetophila |
Synonyms | Comment | Organism |
---|---|---|
AMP-dependent ACS | - |
Methanothrix thermoacetophila |
AMP-forming acetyl-CoA synthetase | - |
Methanothrix thermoacetophila |
Mthe_1194 | locus name | Methanothrix thermoacetophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Methanothrix thermoacetophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
30 min, 15% loss of activity | Methanothrix thermoacetophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Methanothrix thermoacetophila |
General Information | Comment | Organism |
---|---|---|
physiological function | activation of acetate in energy metabolism | Methanothrix thermoacetophila |