General Stability | Organism |
---|---|
ATP and glycerol stabilize during storage at 0-4°C | Bradyrhizobium japonicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Bradyrhizobium japonicum | |
dicarbonic acid diethyl ester | - |
Bradyrhizobium japonicum | |
diphosphate | - |
Bradyrhizobium japonicum | |
FMN | - |
Bradyrhizobium japonicum | |
long-chain acyl-CoA compounds | strong | Bradyrhizobium japonicum | |
NaCl | concentration of 5-20 mM decrease the activity 20-25% | Bradyrhizobium japonicum | |
pyridoxal 5'-phosphate | - |
Bradyrhizobium japonicum | |
pyruvate | - |
Bradyrhizobium japonicum | |
Short-chain CoA esters | - |
Bradyrhizobium japonicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.146 | - |
acetate | - |
Bradyrhizobium japonicum | |
0.202 | - |
CoA | - |
Bradyrhizobium japonicum | |
0.275 | - |
ATP | - |
Bradyrhizobium japonicum | |
0.4 | - |
ADP | - |
Bradyrhizobium japonicum | |
2.3 | - |
dATP | - |
Bradyrhizobium japonicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Km for CaCl2 is 1.2 mM | Bradyrhizobium japonicum | |
Ca2+ | can replace Mg2+ in activation, with 50% of the efficiency relative to MgCl2 | Bradyrhizobium japonicum | |
Co2+ | can replace Mg2+ in activation, 50% as effective as MgCl2, Km for CoCl2 is 0.2 mM | Bradyrhizobium japonicum | |
K+ | KCl increases the activity of the enzyme about 60% at 5 mM and 80% at 20 mM | Bradyrhizobium japonicum | |
Mg2+ | required | Bradyrhizobium japonicum | |
Mg2+ | inhibition at high concentrations where the metal is present as the free ion | Bradyrhizobium japonicum | |
Mg2+ | Km for MgCl2 is 0.3 mM | Bradyrhizobium japonicum | |
Mn2+ | can replace Mg2+ in activation | Bradyrhizobium japonicum | |
Mn2+ | Km for MnCl2 is 0.5 mM | Bradyrhizobium japonicum | |
NH4+ | increases the activity by 30% at 5 mM and 70% at 20 mM | Bradyrhizobium japonicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
2 * 72000, SDS-PAGE | Bradyrhizobium japonicum |
150000 | - |
gel filtration | Bradyrhizobium japonicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bradyrhizobium japonicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bradyrhizobium japonicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bacteroid | from Glycine max L. cv. Williams inoculated with Bradyrhizobium japonicum | Bradyrhizobium japonicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.1 | - |
- |
Bradyrhizobium japonicum |
Storage Stability | Organism |
---|---|
0-4°C, 20 mM Tris/Hcl, pH 8.0, 10 mM ATP, 5 mM MgCl2, 1 mM DTT or 5 mM 2-mercaptoethanol, 20% glycerol, stable for at least 1 month | Bradyrhizobium japonicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + acetate + CoA | 20% of the activity relative to ATP | Bradyrhizobium japonicum | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | - |
Bradyrhizobium japonicum | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + propanoate + CoA | very poor activity | Bradyrhizobium japonicum | AMP + diphosphate + propanoyl-CoA | - |
? | |
dATP + acetate + CoA | 30% of the activity relative to ATP | Bradyrhizobium japonicum | dAMP + diphosphate + acetyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 72000, SDS-PAGE | Bradyrhizobium japonicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bradyrhizobium japonicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
5 min, 20% loss of activity | Bradyrhizobium japonicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bradyrhizobium japonicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | 7.5: about 50% of maximal activity, 10.0: about 70% of maximal activity | Bradyrhizobium japonicum |