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Literature summary for 6.2.1.1 extracted from
- Characterization of the acetyl-CoA synthetase of Acetobacter aceti (1976), Biochim. Biophys. Acta, 450, 410-417.
General Stability
| General Stability | Organism |
|---|---|
| denatured upon freezing | Acetobacter aceti |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenylate | - |
Acetobacter aceti |  |
| AMP | - |
Acetobacter aceti | |
| diphosphate | poor | Acetobacter aceti | |
| erythrose 4-phosphate | - |
Acetobacter aceti | |
| glyceraldehyde 3-phosphate | weak | Acetobacter aceti | |
| glyoxylate | - |
Acetobacter aceti | |
| Xylulose 5-phosphate | - |
Acetobacter aceti | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.104 | - |
CoA | - |
Acetobacter aceti | |
| 0.25 | - |
acetate | - |
Acetobacter aceti | |
| 0.36 | - |
ATP | - |
Acetobacter aceti | |
| 9 | - |
propanoate | - |
Acetobacter aceti | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | absolute requirement for a monovalent cation, stimulates, Km: 14.3 mM, inhibition above 0.1 M | Acetobacter aceti | |
| Mg2+ | required | Acetobacter aceti | |
| Mg2+ | Km: 0.9 mM | Acetobacter aceti | |
| Mg2+ | inhibition above 7 mM | Acetobacter aceti | |
| Mn2+ | 5 mM, 94% of the activation relative to Mg2+ | Acetobacter aceti | |
| Na+ | absolute requirement for a monovalent cation, poor activator, Km: 33 mM, no inhibition at higher concentrations | Acetobacter aceti | |
| NH4+ | absolute requirement for a monovalent cation, stimulates, Km: 14.3 mM, inhibition above 0.1 M | Acetobacter aceti | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
gel filtration | Acetobacter aceti |
| 77000 | - |
x * 77000, SDS-PAGE | Acetobacter aceti |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetobacter aceti | - |
strain ATCC 8554 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetobacter aceti |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Acetobacter aceti |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.1 M buffer, pH 7.5, 3 mM mercaptoethanol, stable for several weeks | Acetobacter aceti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate + CoA | highly specific for ATP | Acetobacter aceti | AMP + diphosphate + acetyl-CoA | - |
? | |
| ATP + acrylate + CoA | - |
Acetobacter aceti | AMP + diphosphate + acryloyl-CoA | - |
? | |
| ATP + butyrate + CoA | no activity | Acetobacter aceti | AMP + diphosphate + butyryl-CoA | - |
? | |
| ATP + propanoate + CoA | - |
Acetobacter aceti | AMP + diphosphate + propanoyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | x * 77000, SDS-PAGE | Acetobacter aceti |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Acetobacter aceti |
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