Literature summary for 6.1.2.1 extracted from

Bugg, T.D.; Wright, G.D.; Dutka-Malen, S.; Arthur, M.; Courvalin, P.; Walsh, C.T.
Molecular basis for vancomycin resistance in Enterococcus faecium BM4147: biosynthesis of a depsipeptide peptidoglycan precursor by vancomycin resistance proteins VanH and VanA (1991), Biochemistry, 30, 10408-10415.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.6	-	D-2-hydroxybutanoate	pH 8.6, 25°C	Enterococcus faecium
3.2	-	D-2-hydroxyvalerate	pH 8.6, 25°C	Enterococcus faecium
7.1	-	D-lactate	pH 8.6, 25°C	Enterococcus faecium
38	-	D-Ala	pH 8.6, 25°C	Enterococcus faecium

Organism

Organism	UniProt	Comment	Textmining
Enterococcus faecium	-	BM4147	-
Enterococcus faecium BM4147	-	BM4147	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Ala + D-2-hydroxybutanoate + ATP	-	Enterococcus faecium	D-Ala-D-2-hydroxybutanoate + ADP + phosphate	-	?
D-Ala + D-2-hydroxybutanoate + ATP	-	Enterococcus faecium BM4147	D-Ala-D-2-hydroxybutanoate + ADP + phosphate	-	?
D-Ala + D-2-hydroxyvalerate + ATP	-	Enterococcus faecium	D-Ala-D-2-hydroxyvalerate + ADP + phosphate	-	?
D-Ala + D-2-hydroxyvalerate + ATP	-	Enterococcus faecium BM4147	D-Ala-D-2-hydroxyvalerate + ADP + phosphate	-	?
D-Ala + D-Ala + ATP	-	Enterococcus faecium	D-Ala-D-Ala + ADP + phosphate	-	?
D-Ala + D-Ala + ATP	-	Enterococcus faecium BM4147	D-Ala-D-Ala + ADP + phosphate	-	?
D-Ala + D-lactate + ATP	-	Enterococcus faecium	D-Ala-D-lactate + ADP + phosphate	-	?
D-Ala + D-lactate + ATP	-	Enterococcus faecium BM4147	D-Ala-D-lactate + ADP + phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.57	-	D-lactate	pH 8.6, 25°C	Enterococcus faecium
1.8	-	D-2-hydroxybutanoate	pH 8.6, 25°C	Enterococcus faecium
2.6	-	D-2-hydroxyvalerate	pH 8.6, 25°C	Enterococcus faecium
4.92	-	D-Ala	pH 8.6, 25°C	Enterococcus faecium

General Information

General Information	Comment	Organism
physiological function	VanA is a D-alanine:D-alanine ligase of altered substrate specificity. VanA catalyzes ester bond formation between D-alanine and the D-hydroxy acid products of VanH, the best substrate being D-2-hydroxybutyrate. The VanA product D-alanyl-D-2-hydroxybutyrate can then be incorporated into the UDPMurNAc-pentapeptide peptidoglycan precursor	Enterococcus faecium

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.13	-	D-Ala	pH 8.6, 25°C	Enterococcus faecium
0.22	-	D-lactate	pH 8.6, 25°C	Enterococcus faecium
0.81	-	D-2-hydroxyvalerate	pH 8.6, 25°C	Enterococcus faecium
3	-	D-2-hydroxybutanoate	pH 8.6, 25°C	Enterococcus faecium

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Release 2023.1 (January 2023)