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Literature summary for 6.1.2.1 extracted from
- Purification and characterization of the VanB ligase associated with type B vancomycin resistance in Enterococcus faecalis V583 (1994), FEBS Lett., 354, 140-142.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| epxression in Escherichia coli | Enterococcus faecalis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
D-Ala | cosubstrate D-Ala, binding of N-terminal Ala residue. pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis |  |
| 3 | - |
D-2-hydroxybutanoate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
| 8.3 | - |
D-2-hydroxyvalerate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
| 11.4 | - |
D-lactate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
| 34 | - |
D-Ala | cosubstrate D-Ala, binding of C-terminal Ala residue. pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
x * 38000, SDS-PAGE | Enterococcus faecalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecalis | - |
V583, isoform VanB | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Ala + D-2-hydroxybutanoate + ATP | - |
Enterococcus faecalis | D-Ala-D-2-hydroxybutanoate + ADP + phosphate | - |
? | |
| D-Ala + D-2-hydroxyvalerate + ATP | - |
Enterococcus faecalis | D-Ala-D-2-hydroxyvalerate + ADP + phosphate | - |
? | |
| D-Ala + D-Ala + ATP | - |
Enterococcus faecalis | D-Ala-D-Ala + ADP + phosphate | - |
? | |
| D-Ala + D-lactate + ATP | - |
Enterococcus faecalis | D-Ala-D-lactate + ADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, SDS-PAGE | Enterococcus faecalis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
D-2-hydroxybutanoate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
| 0.42 | - |
D-2-hydroxyvalerate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
| 0.47 | - |
D-lactate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
| 4.1 | - |
D-Ala | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0414 | - |
D-lactate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
| 0.05 | - |
D-2-hydroxyvalerate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
| 0.084 | - |
D-2-hydroxybutanoate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
| 0.12 | - |
D-Ala | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
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