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Literature summary for 6.1.1.9 extracted from

  • Santamaria-Gomez, J.; Ochoa de Alda, J.A.; Olmedo-Verd, E.; Bru-Martinez, R.; Luque, I.
    Sub-cellular localization and complex formation by aminoacyl-tRNA synthetases in cyanobacteria evidence for interaction of membrane-anchored ValRS with ATP synthase (2016), Front. Microbiol., 7, 857 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.9

Cloned(Commentary)

Cloned (Comment) Organism
gene valS, phylogenetic analysis, recombinant expression of the enzyme in Escherichia coli strain C41(DE3), recombinant expression of GFP-tagged enzyme in Anabaena sp. in membranes Nostoc sp. PCC 7120 = FACHB-418

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information out of the 20 aaRSs, only ValRS is found to localize in thylakoid membranes whereas the other enzymes occupy the soluble portion of the cytoplasm, analysis of aminoacyl-tRNA synthetases protein interactions, overview Nostoc sp. PCC 7120 = FACHB-418
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thylakoid membrane ValRS is anchored to thylakoid membranes by means of the CAAD domain Nostoc sp. PCC 7120 = FACHB-418 42651
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Nostoc sp. PCC 7120 = FACHB-418

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-valine + tRNAVal Nostoc sp. PCC 7120 = FACHB-418
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 AMP + diphosphate + L-valyl-tRNAVal
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 ?
additional information Nostoc sp. PCC 7120 = FACHB-418 interaction of membrane-anchored ValRS with ATP synthase, analysis of the impact of the interaction on the activity of ValRS by measuring aminoacylation activity in whole cell extracts of the wild-type strain and the Anabaena 1C mutant, where the interaction between ValRS and ATP synthase is not expected to occur, analysis of aminoacyl-tRNA synthetases protein interactions, overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Nostoc sp. PCC 7120 = FACHB-418 Q8YX97 i.e. Anabaena sp. {PCC 7120}, or SAG 25.82, or UTEX 2576
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Purification (Commentary)

Purification (Comment) Organism
native enzyme from chloroplast membranes by ultracentrifugation, solubilization with 1%n-dodecyl-beta-D-maltoside, and again ultracentrifugation, recombinant enzyme from Escherichia coli strain C41(DE3), recombinant GFP-tagged enzyme from Anabaena sp. membranes Nostoc sp. PCC 7120 = FACHB-418

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-valine + tRNAVal
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 Nostoc sp. PCC 7120 = FACHB-418 AMP + diphosphate + L-valyl-tRNAVal
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 ?
additional information interaction of membrane-anchored ValRS with ATP synthase, analysis of the impact of the interaction on the activity of ValRS by measuring aminoacylation activity in whole cell extracts of the wild-type strain and the Anabaena 1C mutant, where the interaction between ValRS and ATP synthase is not expected to occur, analysis of aminoacyl-tRNA synthetases protein interactions, overview Nostoc sp. PCC 7120 = FACHB-418 ?
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 ?

Synonyms

Synonyms Comment Organism
ValRS
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 Nostoc sp. PCC 7120 = FACHB-418
valS
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 Nostoc sp. PCC 7120 = FACHB-418
valyl-tRNAsynthetase
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 Nostoc sp. PCC 7120 = FACHB-418

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Nostoc sp. PCC 7120 = FACHB-418

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Nostoc sp. PCC 7120 = FACHB-418

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Nostoc sp. PCC 7120 = FACHB-418

General Information

General Information Comment Organism
evolution the enzyme belongs to the class I aminoacyl-tRNA synthetases Nostoc sp. PCC 7120 = FACHB-418
additional information analysis of aminoacyl-tRNA synthetases protein interactions, overview. The Anabaena sp. ValRs is a CAAD-containing aaRSs, the CAAD domain possesses an inherent membrane targeting ability Nostoc sp. PCC 7120 = FACHB-418
physiological function in Anabaena sp. PCC 7120, ValRS acts as a hub for the nucleation of a translational complex by attracting other aaRSs to the membranes Nostoc sp. PCC 7120 = FACHB-418