Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae as a His-tagged fusion protein | Homo sapiens |
expressed in Saccharomyces cerevisiae as a His-tagged fusion protein | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-97 | an N-domain-deleted yeast valyltRNA synthetase mutant (DELTA1-97) form Saccharomyces cerevisiae can be rescued by fusion of the equivalent domain from its human homologue | Homo sapiens |
DELTA1-97 | deletion of N-terminal polypeptide extension of 97 residues, which is absent in bacteria, severely impairs tRNA binding, aminoacylation, and complementation activities of the enzyme. This N-domain-deleted yeast valyl-tRNA synthetase mutant can be rescued by fusion of the equivalent domain from its human homologue | Saccharomyces cerevisiae |
DELTA32-71 | deletion of a lysine rich insert impairs aminoacylation activity of the enzyme in vitro but aminoacylation activity is still significantly higher than in DELTA1-97 mutant. Km: 0.001 mM (L-valyl-tRNAVal), kcast: 0.06/sec (L-valyl-tRNAVal) | Saccharomyces cerevisiae |
additional information | fusion of the N-terminal 97 residue domain of human ValRS to Escherichia coli glutaminyl-tRNA synthetase enables the otherwise inactive prokaryotic enzyme to function as a yeast enzyme in vivo. Different from the native yeast enzyme, which shows different affinities toward mixed tRNA populations, the fusion enzyme exhibites similar binding affinities for all yeast tRNAs | Homo sapiens |
additional information | fusion of the N-terminal 97 residue domain of the yeast enzyme or its human counterpart to Escherichia coli glutaminyl-tRNA synthetase enables the otherwise inactive prokaryotic enzyme to function as a yeast enzyme in vivo. Different from the native yeast enzyme, which shows different affinities toward mixed tRNA populations, the fusion enzyme exhibites similar binding affinities for all yeast tRNAs | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
L-valyl-tRNAVal | - |
Saccharomyces cerevisiae | |
0.001 | - |
L-valyl-tRNAVal | DELTA32-71 mutant | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Homo sapiens |
using Ni-NTA chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-valine + tRNAVal | - |
Homo sapiens | AMP + diphosphate + L-valyl-tRNAVal | - |
? | |
ATP + L-valine + tRNAVal | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-valyl-tRNAVal | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ValRS | - |
Homo sapiens |
ValRS | - |
Saccharomyces cerevisiae |
Valyl-tRNA synthetase | - |
Homo sapiens |
Valyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
L-valyl-tRNAVal | DELTA32-71 mutant | Saccharomyces cerevisiae | |
0.2 | - |
L-valyl-tRNAVal | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Homo sapiens |
7.9 | - |
assay at | Saccharomyces cerevisiae |