KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
L-valine | with mutant tRNAVal with 3'-terminal isoguanosine base analogue, pH 7.5, 37°C | Escherichia coli | |
0.0043 | - |
L-valine | with wild-type tRNAVal, pH 7.5, 37°C | Escherichia coli | |
0.0048 | - |
L-valine | with mutant tRNAVal with 3'-terminal inosine base analogue, pH 7.5, 37°C | Escherichia coli | |
0.005 | - |
L-valine | with mutant tRNAVal with 3'-terminal 2-aminopurine base analogue, pH 7.5, 37°C | Escherichia coli | |
0.0088 | - |
L-valine | with mutant tRNAVal with 3'-terminal purine riboside base analogue, pH 7.5, 37°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-valine + tRNAVal | Escherichia coli | - |
AMP + diphosphate + L-valyl-tRNAVal | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
catalytic activity with L-valine and diverse tRNA analogues with functional group substitutions at the terminal nucleoside of the 3'-end, overview | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-valine + tRNAVal | - |
Escherichia coli | AMP + diphosphate + L-valyl-tRNAVal | - |
? | |
ATP + L-valine + tRNAVal,3' 2-aminopurine | mutant tRNAVal with 3'-terminal 2-aminopurine base analogue substitution | Escherichia coli | AMP + diphosphate + L-valyl-tRNAVal, 3' 2-aminopurine | - |
? | |
ATP + L-valine + tRNAVal,3' inosine | mutant tRNAVal with 3'-terminal inosine base analogue substitution | Escherichia coli | AMP + diphosphate + L-valyl-tRNAVal, 3' inosine | - |
? | |
ATP + L-valine + tRNAVal,3' isoguanosine | mutant tRNAVal with 3'-terminal isoguanosine base analogue substitution | Escherichia coli | AMP + diphosphate + L-valyl-tRNAVal, 3' isoguanosine | - |
? | |
ATP + L-valine + tRNAVal,3' purine riboside | mutant tRNAVal with 3'-terminal purine riboside base analogue substitution | Escherichia coli | AMP + diphosphate + L-valyl-tRNAVal, 3' purine riboside | - |
? | |
additional information | catalytic activity with L-valine and diverse tRNA analogues with functional group substitutions at the terminal nucleoside of the 3'-end, some of which stimulate the editing reaction, overview | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G7a | - |
Escherichia coli |
Synthetase, valyl-transfer ribonucleate | - |
Escherichia coli |
Valine transfer ribonucleate ligase | - |
Escherichia coli |
Valine translase | - |
Escherichia coli |
Valine--tRNA ligase | - |
Escherichia coli |
ValRS | - |
Escherichia coli |
Valyl transfer ribonucleic acid synthetase | - |
Escherichia coli |
Valyl-transfer ribonucleate synthetase | - |
Escherichia coli |
Valyl-transfer RNA synthetase | - |
Escherichia coli |
Valyl-tRNA ligase | - |
Escherichia coli |
Valyl-tRNA synthetase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.68 | - |
L-valine | with mutant tRNAVal with 3'-terminal 2-aminopurine base analogue, pH 7.5, 37°C | Escherichia coli | |
0.99 | - |
L-valine | with mutant tRNAVal with 3'-terminal isoguanosine base analogue, pH 7.5, 37°C | Escherichia coli | |
1.06 | - |
L-valine | with mutant tRNAVal with 3'-terminal inosine base analogue, pH 7.5, 37°C | Escherichia coli | |
6.08 | - |
L-valine | with mutant tRNAVal with 3'-terminal 2-aminopurine base analogue, pH 7.5, 37°C | Escherichia coli | |
6.08 | - |
L-valine | with mutant tRNAVal with 3'-terminal inosine base analogue, pH 7.5, 37°C | Escherichia coli | |
6.08 | - |
L-valine | with mutant tRNAVal with 3'-terminal isoguanosine base analogue, pH 7.5, 37°C | Escherichia coli | |
13.9 | - |
L-valine | with wild-type tRNAVal, pH 7.5, 37°C | Escherichia coli | |
14.4 | - |
L-valine | with mutant tRNAVal with 3'-terminal purine riboside base analogue, pH 7.5, 37°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |