Cloned (Comment) | Organism |
---|---|
gene PH0574, expression in Escherichia coli strain BL21 | Pyrococcus horikoshii |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and selenomethionine-labeld AlaRS editing-domain homolog, PH0574, 37.6 mg/ml protein, 27.5%w/v PEG 4000, and 100 mM MES-Na, pH 6.3, using a full-automatic protein crystallization and observation system, X-ray diffraction structure determination and analysis at 1.45 A resolution | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pyrococcus horikoshii | |
Zn2+ | active site bound by His9, His13, His120, and Cys116, involved in the catalytic reaction | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18152 | - |
1 * 18152, crystal structure analysis, two-domain structure consisting of seven antiparallel beta-sheets and six helices | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + tRNAAla | Pyrococcus horikoshii | - |
AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
ATP + L-alanine + tRNAAla | Pyrococcus horikoshii OT-3 | - |
AMP + diphosphate + L-alanyl-tRNAAla | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O58307 | strain OT3, gene PH0574 | - |
Pyrococcus horikoshii OT-3 | O58307 | strain OT3, gene PH0574 | - |
Purification (Comment) | Organism |
---|---|
recombinant PH0574, from Escherichia coli strain BL21 by two steps of anion exchange chromatography, hydroxyapatite chromatography, and gel filtration | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + tRNAAla | - |
Pyrococcus horikoshii | AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
ATP + L-alanine + tRNAAla | AlaRS hydrolyzes mischarged tRNA by the catalytic zinc ion in the editing domain, modeling, overview | Pyrococcus horikoshii | AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
ATP + L-alanine + tRNAAla | - |
Pyrococcus horikoshii OT-3 | AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
ATP + L-alanine + tRNAAla | AlaRS hydrolyzes mischarged tRNA by the catalytic zinc ion in the editing domain, modeling, overview | Pyrococcus horikoshii OT-3 | AMP + diphosphate + L-alanyl-tRNAAla | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 18152, crystal structure analysis, two-domain structure consisting of seven antiparallel beta-sheets and six helices | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
Alanyl-tRNA synthetase | - |
Pyrococcus horikoshii |
AlaRS | - |
Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pyrococcus horikoshii |