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Literature summary for 6.1.1.7 extracted from
- tmRNA from Thermus thermophilus. Interaction with alanyl-tRNA synthetase and elongation factor Tu (2003), Eur. J. Biochem., 270, 463-475.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | elongation factor Tu protects acylated tRNAAla against hydrolysis of the alanyl ester bond | Thermus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| elongation factor Tu | strongly inhibits the aminoacylation of tmRNA, deviation from the mechanism of aminoacyl ester bond protection upon formation of the canonical ternary complx between the elongation gactor Tu, nucleotide cofactor and aminoacyl-tRNA | Thermus thermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Thermus thermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 195000 | - |
gel filtration | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-alanine + tRNAAla | Thermus thermophilus | - |
AMP + diphosphate + L-alanyl-tRNAAla | - |
? |  |
| ATP + L-alanine + tRNAAla | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
- |
- |
| Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.105 | - |
purified enzyme | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-alanine + tmRNA | recombinant transfer messenger RNA substrate from Thermus thermophilus expressed in Escherichia coli | Thermus thermophilus | AMP + diphosphate + L-alanyl-tmRNA | - |
? | |
| ATP + L-alanine + tmRNA | recombinant transfer messenger RNA substrate from Thermus thermophilus expressed in Escherichia coli | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-alanyl-tmRNA | - |
? | |
| ATP + L-alanine + tRNAAla | - |
Thermus thermophilus | AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
| ATP + L-alanine + tRNAAla | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ala-tRNA synthetase | - |
Thermus thermophilus |
| Alanine transfer RNA synthetase | - |
Thermus thermophilus |
| Alanine translase | - |
Thermus thermophilus |
| Alanine tRNA synthetase | - |
Thermus thermophilus |
| Alanine-transfer RNA ligase | - |
Thermus thermophilus |
| alanine-tRNA ligase | - |
Thermus thermophilus |
| Alanyl-transfer ribonucleate synthetase | - |
Thermus thermophilus |
| Alanyl-transfer ribonucleic acid synthetase | - |
Thermus thermophilus |
| Alanyl-transfer RNA synthetase | - |
Thermus thermophilus |
| Alanyl-tRNA synthetase | - |
Thermus thermophilus |
| AlaRS | - |
Thermus thermophilus |
| Synthase, alanyl-transfer ribonucleate | - |
Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Thermus thermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 75 | half-maximal activity at 25°C and 75°C with substrate tRNAAla, a sharp 345fold drop of activity occurs at 70°C, temperature profile | Thermus thermophilus |
| 35 | 65 | half-maximal activity at 35°C and 65°C with substrate tmRNA, a sharp 345fold drop of activity occurs at 40°C, temperature profile | Thermus thermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
tmRNA | pH 7.6, 50°C | Thermus thermophilus | |
| 2.2 | - |
tRNAAla | pH 7.6, 50°C | Thermus thermophilus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Thermus thermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Thermus thermophilus | |
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Release 2023.1 (January 2023)
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