Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.7 extracted from

  • Nishio, K.; Kawakami, M.
    Purification and properties of alanyl-tRNA synthetase from Bombyx mori. A monomeric enzyme (1984), J. Biochem., 96, 1867-1874.
    View publication on PubMed
Search for more literature for 6.1.1.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 tRNAAla
-
 Bombyx mori
0.3
-
 ATP
-
 Bombyx mori
0.8
-
 L-alanine
-
 Bombyx mori

Metals/Ions

Metals/Ions Comment Organism Structure
KCl optimum concentration: 50-100 mM Bombyx mori
Mg2+ 6-16 mM Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
 1 * 115000, SDS-PAGE, gel filtration in 6 M guanidine-HCl Bombyx mori
116000
-
 gel filtration Bombyx mori

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
silk gland posterior Bombyx mori
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.65
-
-
 Bombyx mori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-alanine + tRNAAla
-
 Bombyx mori AMP + diphosphate + L-alanyl-tRNAAla
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 115000, SDS-PAGE, gel filtration in 6 M guanidine-HCl Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 8.4
-
 Bombyx mori