Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
205000 | - |
size exclusion chromatography | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | P74941 | overproduced in Escherichia coli | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | P74941 | overproduced in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + tRNAAla | - |
Thermus thermophilus | AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
ATP + L-alanine + tRNAAla | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
additional information | alanine dependent ATP-diphosphate exchange: alanine + ATP + enzyme /alanine-AMP-enzyme + diphosphate | Thermus thermophilus | ? | - |
? | |
additional information | alanine dependent ATP-diphosphate exchange: alanine + ATP + enzyme /alanine-AMP-enzyme + diphosphate | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
aminoacylation | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
stable up to | Thermus thermophilus |
85 | - |
denaturation at | Thermus thermophilus |