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Literature summary for 6.1.1.7 extracted from

  • Lechler, A.; Martin, A.; Zuleeg, T.; Limmer, S.; Kreutzer, R.
    A biologically active 53 kDa fragment of overproduced alanyl-tRNA synthetase from Thermus thermophilus HB8 specifically interacts with tRNAAla acceptor helix (1997), Nucleic Acids Res., 25, 2737-2744.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
205000
-
size exclusion chromatography Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus P74941 overproduced in Escherichia coli
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 P74941 overproduced in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-alanine + tRNAAla
-
Thermus thermophilus AMP + diphosphate + L-alanyl-tRNAAla
-
?
ATP + L-alanine + tRNAAla
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-alanyl-tRNAAla
-
?
additional information alanine dependent ATP-diphosphate exchange: alanine + ATP + enzyme /alanine-AMP-enzyme + diphosphate Thermus thermophilus ?
-
?
additional information alanine dependent ATP-diphosphate exchange: alanine + ATP + enzyme /alanine-AMP-enzyme + diphosphate Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
aminoacylation Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
stable up to Thermus thermophilus
85
-
denaturation at Thermus thermophilus