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Literature summary for 6.1.1.6 extracted from

  • Baleva, M.V.; Meyer, M.; Entelis, N.; Tarassov, I.; Kamenski, P.; Masquida, B.
    Factors beyond enolase 2 and mitochondrial lysyl-tRNA synthetase precursor are required for tRNA import into yeast mitochondria (2017), Biochemistry (Moscow), 82, 1324-1335 .
    View publication on PubMed
Search for more literature for 6.1.1.6

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-lysine + tRNALys Saccharomyces cerevisiae
-
 AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys Saccharomyces cerevisiae YPH499
-
 AMP + diphosphate + L-lysyl-tRNALys
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P32048
-
-
Saccharomyces cerevisiae YPH499 P32048
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-lysine + tRNALys
-
 Saccharomyces cerevisiae AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys
-
 Saccharomyces cerevisiae YPH499 AMP + diphosphate + L-lysyl-tRNALys
-
 ?

Synonyms

Synonyms Comment Organism
FOSTERSO_4045
-
 Saccharomyces cerevisiae
Lysyl-tRNA synthetase
-
 Saccharomyces cerevisiae
mitochondrial lysyl-tRNA synthetase
-
 Saccharomyces cerevisiae
Msk1p
-
 Saccharomyces cerevisiae
preMsk1p
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function in yeast, the import of tRNALys with CUU anticodon (tRK1) relies on a complex mechanism where interaction with enolase 2 (Eno2p) dictates a deep conformational change of the tRNA. This event is believed to mask the tRNA from the cytosolic translational machinery to redirect it towards the mitochondria. Once near the mitochondrial outer membrane, the precursor of the mitochondrial lysyl-tRNA synthetase (preMsk1p) takes over enolase to carry the tRNA within the mitochondrial matrix, where it is supposed to participate in translation following correct refolding. Mechanism of mitochondrial import of the yeast lysine isoacceptor tRNA with a CUU anticodon (tRK1). tRK1 in the canonical Lform undertakes refolding (Fform) through interaction mainly with enolase 2 (Eno2p), overview. While about 95% of the tRK1 cellular pool is used in cytosolic translation, this complex shuttles the remaining about 5% to the mitochondrial surface, where it interacts with preMsk1p, which proceeds to import properly. The mitochondrial targeting sequence (MTS) is displayed as an additional appendage bound to the N-terminal end of the protein representation Saccharomyces cerevisiae