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Literature summary for 6.1.1.6 extracted from
- Structural analysis of malaria-parasite lysyl-tRNA synthetase provides a platform for drug development (2013), Acta Crystallogr. Sect. D, 69, 785-795.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Plasmodium falciparum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 0.1 M Bis-Tris pH 6.5, 2% (v/v) tascimate pH 6.0, 20% (w/v) PEG 3350 | Plasmodium falciparum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Plasmodium falciparum | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
2 * 65000, SDS-PAGE | Plasmodium falciparum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-lysine + tRNALys | Plasmodium falciparum | - |
AMP + diphosphate + L-lysyl-tRNALys | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | Q8IDJ8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| amylose bead chromatography and S200 gel filtration | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-lysine + tRNALys | - |
Plasmodium falciparum | AMP + diphosphate + L-lysyl-tRNALys | - |
? | |
| additional information | the enzyme is also capable of synthesizing the signalling molecule diadenosine tetraphosphate using ATP as a substrate in the presence of L-Lys and Zn2+ | Plasmodium falciparum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 65000, SDS-PAGE | Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| KRS-1 | - |
Plasmodium falciparum |
| LysRS | - |
Plasmodium falciparum |
| Lysyl-tRNA synthetase | - |
Plasmodium falciparum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Plasmodium falciparum | |
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