Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00074 | - |
tRNALys | at 25°C, in 250 mM KCl, 100 mM HEPES, pH 7.5, 10 mM dithiothreitol, 10 mM MgCl2, 0.05 mg/ml bovine serum albumin | Methanothermobacter thermautotrophicus | |
0.0066 | - |
L-lysine | at 25°C, in 250 mM KCl, 100 mM HEPES, pH 7.5, 10 mM dithiothreitol, 10 mM MgCl2, 0.05 mg/ml bovine serum albumin | Methanothermobacter thermautotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sephacryl S300 26/60 column chromatography and HiPrep 16/10 Q Sepharose FF column chromatography | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | - |
Methanothermobacter thermautotrophicus | AMP + L-lysyl-tRNALys + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LysRS1 | - |
Methanothermobacter thermautotrophicus |
Lysyl-tRNA synthetase | - |
Methanothermobacter thermautotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0143 | - |
L-lysine | at 25°C, in 250 mM KCl, 100 mM HEPES, pH 7.5, 10 mM dithiothreitol, 10 mM MgCl2, 0.05 mg/ml bovine serum albumin | Methanothermobacter thermautotrophicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methanothermobacter thermautotrophicus |