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Literature summary for 6.1.1.6 extracted from

  • Praetorius-Ibba, M.; Hausmann, C.D.; Paras, M.; Rogers, T.E.; Ibba, M.
    Functional association between three archaeal aminoacyl-tRNA synthetases (2007), J. Biol. Chem., 282, 3680-3687.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00074
-
tRNALys at 25°C, in 250 mM KCl, 100 mM HEPES, pH 7.5, 10 mM dithiothreitol, 10 mM MgCl2, 0.05 mg/ml bovine serum albumin Methanothermobacter thermautotrophicus
0.0066
-
L-lysine at 25°C, in 250 mM KCl, 100 mM HEPES, pH 7.5, 10 mM dithiothreitol, 10 mM MgCl2, 0.05 mg/ml bovine serum albumin Methanothermobacter thermautotrophicus

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sephacryl S300 26/60 column chromatography and HiPrep 16/10 Q Sepharose FF column chromatography Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-lysine + tRNALys
-
Methanothermobacter thermautotrophicus AMP + L-lysyl-tRNALys + diphosphate
-
?

Synonyms

Synonyms Comment Organism
LysRS1
-
Methanothermobacter thermautotrophicus
Lysyl-tRNA synthetase
-
Methanothermobacter thermautotrophicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0143
-
L-lysine at 25°C, in 250 mM KCl, 100 mM HEPES, pH 7.5, 10 mM dithiothreitol, 10 mM MgCl2, 0.05 mg/ml bovine serum albumin Methanothermobacter thermautotrophicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Methanothermobacter thermautotrophicus