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Literature summary for 6.1.1.6 extracted from

  • Levengood, J.D.; Roy, H.; Ishitani, R.; Soell, D.; Nureki, O.; Ibba, M.
    Anticodon recognition and discrimination by the alpha-helix cage domain of class I lysyl-tRNA synthetase (2007), Biochemistry, 46, 11033-11038.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3+ RPC) cells Pyrococcus horikoshii

Protein Variants

Protein Variants Comment Organism
Y491E exhibits significant improvement compared to the wild type in aminoacylation of a tRNALysUUG Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.87
-
 tRNALysUUU wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP Pyrococcus horikoshii
1.4
-
 tRNALysCUU wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP Pyrococcus horikoshii
1.5
-
 tRNALysGUU wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
MonoQ 5/5 column chromatography and Superose 12 gel filtration Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-lysine + tRNALysCUU
-
 Pyrococcus horikoshii AMP + L-lysyl-tRNALysCUU + diphosphate
-
 ?
ATP + L-lysine + tRNALysGUU
-
 Pyrococcus horikoshii AMP + L-lysyl-tRNALysGUU + diphosphate
-
 ?
ATP + L-lysine + tRNALysUCU
-
 Pyrococcus horikoshii AMP + L-lysyl-tRNALysUCU + diphosphate
-
 ?
ATP + L-lysine + tRNALysUGU
-
 Pyrococcus horikoshii AMP + L-lysyl-tRNALysUGU + diphosphate
-
 ?
ATP + L-lysine + tRNALysUUC
-
 Pyrococcus horikoshii AMP + L-lysyl-tRNALysUUC + diphosphate
-
 ?
ATP + L-lysine + tRNALysUUG
-
 Pyrococcus horikoshii AMP + L-lysyl-tRNALysUUG + diphosphate
-
 ?
ATP + L-lysine + tRNALysUUU
-
 Pyrococcus horikoshii AMP + L-lysyl-tRNALysUUU + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
class I lysyl-tRNA synthetase
-
 Pyrococcus horikoshii
LysRS1
-
 Pyrococcus horikoshii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.043
-
 tRNALysGUU wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP Pyrococcus horikoshii
0.062
-
 tRNALysCUU wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP Pyrococcus horikoshii
0.44
-
 tRNALysUUU wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP Pyrococcus horikoshii

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Pyrococcus horikoshii