Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes, expression in Escherichia coli BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E240D | 1.2fold increase in Km-value for Lys, 21fold decrease in turnover number for Lys, Km-value for ATP is nearly identical to wild-type value, 24fold decrease in turnover number for ATP | Escherichia coli |
E240Q | 1.4fold increase in Km-value for Lys, 207fold decrease in turnover number for Lys, 1.2fold increase in Km-value for ATP, 261fold decrease in turnover number for ATP | Escherichia coli |
E278D | 98fold increase in Km-value for Lys, 11fold decrease in turnover number for Lys, 1.3fold increase in Km-value for ATP, 63fold decrease in turnover number for ATP | Escherichia coli |
E278Q | 1.9fold decrease in Km-value for Lys, 120fold decrease in turnover number for Lys, 1.5fold decrease in Km-value for ATP, 200fold decrease in turnover number for ATP | Escherichia coli |
E428D | 8.5fold increase in Km-value for Lys, 1.8fold decrease in turnover number for Lys, 1.7fold increase in Km-value for ATP, 3.4fold decrease in turnover number for ATP | Escherichia coli |
E428Q | 2fold decrease in Km-value for Lys, 9fold decrease in turnover number for Lys, 1.5fold decrease in Km-value for ATP, 200fold decrease in turnover number for ATP | Escherichia coli |
F426H | 78fold increase in Km-value for Lys, 1.3fold decrease in turnover number for Lys, 1.6fold increase in Km-value for ATP, 5fold decrease in turnover number for ATP | Escherichia coli |
F426W | 6.2fold increase in Km-value for Lys, 3fold decrease in turnover number for Lys, 9.2fold increase in Km-value for ATP, 1.4fold decrease in turnover number for ATP | Escherichia coli |
G216A | 75fold increase in Km-value for Lys, 1.9fold increase in turnover number for Lys, 16fold increase in Km-value for ATP, 4.25fold decrease in turnover number for ATP | Escherichia coli |
N424D | 2fold increase in Km-value for Lys, 1.1fold decrease in turnover number for Lys, 1.8fold increase in Km-value for ATP, 3.4fold decrease in turnover number for ATP | Escherichia coli |
N424Q | 20fold increase in Km-value for Lys, 2.8fold decrease in turnover number for Lys, 69fold increase in Km-value for ATP, 1.5fold decrease in turnover number for ATP | Escherichia coli |
Y280F | 9.2fold increase in Km-value for Lys, 58fold decrease in turnover number for Lys, 5.2fold increase in Km-value for ATP, 20fold decrease in turnover number for ATP | Escherichia coli |
Y280S | 44fold increase in Km-value for Lys, 1.1fold decrease in turnover number for Lys, 3.9fold increase in Km-value for ATP, 9.7fold decrease in turnover number for ATP | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | - |
Escherichia coli | |
S-(2-aminoethyl)-L-cysteine | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E428Q | Escherichia coli | |
0.0014 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E278Q | Escherichia coli | |
0.0026 | - |
L-lysine | pH 7.2, 37°C, wild-type enzyme | Escherichia coli | |
0.0033 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E240D | Escherichia coli | |
0.0036 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E240Q | Escherichia coli | |
0.0052 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme N424D | Escherichia coli | |
0.0078 | - |
ATP | pH 7.2, 37°C, mutant enzyme E278Q | Escherichia coli | |
0.0078 | - |
ATP | pH 7.2, 37°C, mutant enzyme E428Q | Escherichia coli | |
0.011 | - |
ATP | pH 7.2, 37°C, mutant enzyme E240D | Escherichia coli | |
0.012 | - |
ATP | pH 7.2, 37°C, wild-type enzyme | Escherichia coli | |
0.014 | - |
ATP | pH 7.2, 37°C, mutant enzyme E240Q | Escherichia coli | |
0.016 | - |
ATP | pH 7.2, 37°C, mutant enzyme E278D | Escherichia coli | |
0.016 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme F426W | Escherichia coli | |
0.019 | - |
ATP | pH 7.2, 37°C, mutant enzyme F426H | Escherichia coli | |
0.02 | - |
ATP | pH 7.2, 37°C, mutant enzyme E428D | Escherichia coli | |
0.021 | - |
ATP | pH 7.2, 37°C, mutant enzyme N424D | Escherichia coli | |
0.022 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E428D | Escherichia coli | |
0.024 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme Y280F | Escherichia coli | |
0.052 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme N424Q | Escherichia coli | |
0.062 | - |
ATP | pH 7.2, 37°C, mutant enzyme Y280F | Escherichia coli | |
0.11 | - |
ATP | pH 7.2, 37°C, mutant enzyme F426W | Escherichia coli | |
0.114 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme Y280S | Escherichia coli | |
0.147 | - |
ATP | pH 7.2, 37°C, mutant enzyme Y280S | Escherichia coli | |
0.195 | - |
ATP | pH 7.2, 37°C, mutant enzyme G216A | Escherichia coli | |
0.196 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme G216A | Escherichia coli | |
0.203 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme F426H | Escherichia coli | |
0.254 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E278D | Escherichia coli | |
0.83 | - |
ATP | pH 7.2, 37°C, mutant enzyme N424Q | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | - |
Escherichia coli | AMP + L-lysyl-tRNALys + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class II lysyl-tRNA synthetase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0087 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E240Q | Escherichia coli | |
0.013 | - |
ATP | pH 7.2, 37°C, mutant enzyme E240Q | Escherichia coli | |
0.015 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E278Q | Escherichia coli | |
0.017 | - |
ATP | pH 7.2, 37°C, mutant enzyme E278Q | Escherichia coli | |
0.017 | - |
ATP | pH 7.2, 37°C, mutant enzyme E428Q | Escherichia coli | |
0.02 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E428D | Escherichia coli | |
0.031 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme Y280F | Escherichia coli | |
0.054 | - |
ATP | pH 7.2, 37°C, mutant enzyme E278D | Escherichia coli | |
0.086 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E240D | Escherichia coli | |
0.14 | - |
ATP | pH 7.2, 37°C, mutant enzyme E240D | Escherichia coli | |
0.16 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E278D | Escherichia coli | |
0.17 | - |
ATP | pH 7.2, 37°C, mutant enzyme Y280F | Escherichia coli | |
0.35 | - |
ATP | pH 7.2, 37°C, mutant enzyme Y280S | Escherichia coli | |
0.6 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme F426W | Escherichia coli | |
0.65 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme N424Q | Escherichia coli | |
0.67 | - |
ATP | pH 7.2, 37°C, mutant enzyme F426H | Escherichia coli | |
0.8 | - |
ATP | pH 7.2, 37°C, mutant enzyme G216A | Escherichia coli | |
1 | - |
ATP | pH 7.2, 37°C, mutant enzyme E428D | Escherichia coli | |
1 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E428D | Escherichia coli | |
1 | - |
ATP | pH 7.2, 37°C, mutant enzyme N424D | Escherichia coli | |
1.6 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme N424D | Escherichia coli | |
1.6 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme Y280S | Escherichia coli | |
1.8 | - |
L-lysine | pH 7.2, 37°C, wild-type enzyme | Escherichia coli | |
2.2 | - |
ATP | pH 7.2, 37°C, mutant enzyme N424Q | Escherichia coli | |
2.4 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme F426H | Escherichia coli | |
2.4 | - |
ATP | pH 7.2, 37°C, mutant enzyme F426W | Escherichia coli | |
3.4 | - |
ATP | pH 7.2, 37°C, wild-type enzyme | Escherichia coli | |
3.4 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme G216A | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme E240Q | Escherichia coli | |
0.000011 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme E428Q | Escherichia coli | |
0.000023 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme E278Q | Escherichia coli | |
0.000028 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, wild-type enzyme | Escherichia coli | |
0.000029 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme E240D | Escherichia coli | |
0.000039 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme N424D | Escherichia coli | |
0.000041 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme E278D | Escherichia coli | |
0.00005 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme F426W | Escherichia coli | |
0.000055 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme N424Q | Escherichia coli | |
0.000059 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme G216A | Escherichia coli | |
0.000061 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme E428D | Escherichia coli | |
0.00022 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme Y280S | Escherichia coli | |
0.00027 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme Y280F | Escherichia coli | |
0.0013 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme F426H | Escherichia coli | |
0.0015 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme E428Q | Escherichia coli | |
0.0031 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme E240D | Escherichia coli | |
0.0039 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, wild-type enzyme | Escherichia coli | |
0.004 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme N424D | Escherichia coli | |
0.005 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme E278Q | Escherichia coli | |
0.0054 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme E240Q | Escherichia coli | |
0.025 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme F426W | Escherichia coli | |
0.034 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme E428D | Escherichia coli | |
0.072 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme Y280F | Escherichia coli | |
0.076 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme N424Q | Escherichia coli | |
0.34 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme Y280S | Escherichia coli | |
1.24 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme G216A | Escherichia coli | |
1.29 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme E278D | Escherichia coli | |
1.31 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme F426H | Escherichia coli |