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Literature summary for 6.1.1.6 extracted from

  • Ataide, S.F.; Ibba, M.
    Discrimination of cognate and noncognate substrates at the active site of class II lysyl-tRNA synthetase (2004), Biochemistry, 43, 11836-11841.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes, expression in Escherichia coli BL21(DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
E240D 1.2fold increase in Km-value for Lys, 21fold decrease in turnover number for Lys, Km-value for ATP is nearly identical to wild-type value, 24fold decrease in turnover number for ATP Escherichia coli
E240Q 1.4fold increase in Km-value for Lys, 207fold decrease in turnover number for Lys, 1.2fold increase in Km-value for ATP, 261fold decrease in turnover number for ATP Escherichia coli
E278D 98fold increase in Km-value for Lys, 11fold decrease in turnover number for Lys, 1.3fold increase in Km-value for ATP, 63fold decrease in turnover number for ATP Escherichia coli
E278Q 1.9fold decrease in Km-value for Lys, 120fold decrease in turnover number for Lys, 1.5fold decrease in Km-value for ATP, 200fold decrease in turnover number for ATP Escherichia coli
E428D 8.5fold increase in Km-value for Lys, 1.8fold decrease in turnover number for Lys, 1.7fold increase in Km-value for ATP, 3.4fold decrease in turnover number for ATP Escherichia coli
E428Q 2fold decrease in Km-value for Lys, 9fold decrease in turnover number for Lys, 1.5fold decrease in Km-value for ATP, 200fold decrease in turnover number for ATP Escherichia coli
F426H 78fold increase in Km-value for Lys, 1.3fold decrease in turnover number for Lys, 1.6fold increase in Km-value for ATP, 5fold decrease in turnover number for ATP Escherichia coli
F426W 6.2fold increase in Km-value for Lys, 3fold decrease in turnover number for Lys, 9.2fold increase in Km-value for ATP, 1.4fold decrease in turnover number for ATP Escherichia coli
G216A 75fold increase in Km-value for Lys, 1.9fold increase in turnover number for Lys, 16fold increase in Km-value for ATP, 4.25fold decrease in turnover number for ATP Escherichia coli
N424D 2fold increase in Km-value for Lys, 1.1fold decrease in turnover number for Lys, 1.8fold increase in Km-value for ATP, 3.4fold decrease in turnover number for ATP Escherichia coli
N424Q 20fold increase in Km-value for Lys, 2.8fold decrease in turnover number for Lys, 69fold increase in Km-value for ATP, 1.5fold decrease in turnover number for ATP Escherichia coli
Y280F 9.2fold increase in Km-value for Lys, 58fold decrease in turnover number for Lys, 5.2fold increase in Km-value for ATP, 20fold decrease in turnover number for ATP Escherichia coli
Y280S 44fold increase in Km-value for Lys, 1.1fold decrease in turnover number for Lys, 3.9fold increase in Km-value for ATP, 9.7fold decrease in turnover number for ATP Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
5'-O-[N-(L-lysyl)sulfamoyl]adenosine
-
Escherichia coli
S-(2-aminoethyl)-L-cysteine
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
L-lysine pH 7.2, 37°C, mutant enzyme E428Q Escherichia coli
0.0014
-
L-lysine pH 7.2, 37°C, mutant enzyme E278Q Escherichia coli
0.0026
-
L-lysine pH 7.2, 37°C, wild-type enzyme Escherichia coli
0.0033
-
L-lysine pH 7.2, 37°C, mutant enzyme E240D Escherichia coli
0.0036
-
L-lysine pH 7.2, 37°C, mutant enzyme E240Q Escherichia coli
0.0052
-
L-lysine pH 7.2, 37°C, mutant enzyme N424D Escherichia coli
0.0078
-
ATP pH 7.2, 37°C, mutant enzyme E278Q Escherichia coli
0.0078
-
ATP pH 7.2, 37°C, mutant enzyme E428Q Escherichia coli
0.011
-
ATP pH 7.2, 37°C, mutant enzyme E240D Escherichia coli
0.012
-
ATP pH 7.2, 37°C, wild-type enzyme Escherichia coli
0.014
-
ATP pH 7.2, 37°C, mutant enzyme E240Q Escherichia coli
0.016
-
ATP pH 7.2, 37°C, mutant enzyme E278D Escherichia coli
0.016
-
L-lysine pH 7.2, 37°C, mutant enzyme F426W Escherichia coli
0.019
-
ATP pH 7.2, 37°C, mutant enzyme F426H Escherichia coli
0.02
-
ATP pH 7.2, 37°C, mutant enzyme E428D Escherichia coli
0.021
-
ATP pH 7.2, 37°C, mutant enzyme N424D Escherichia coli
0.022
-
L-lysine pH 7.2, 37°C, mutant enzyme E428D Escherichia coli
0.024
-
L-lysine pH 7.2, 37°C, mutant enzyme Y280F Escherichia coli
0.052
-
L-lysine pH 7.2, 37°C, mutant enzyme N424Q Escherichia coli
0.062
-
ATP pH 7.2, 37°C, mutant enzyme Y280F Escherichia coli
0.11
-
ATP pH 7.2, 37°C, mutant enzyme F426W Escherichia coli
0.114
-
L-lysine pH 7.2, 37°C, mutant enzyme Y280S Escherichia coli
0.147
-
ATP pH 7.2, 37°C, mutant enzyme Y280S Escherichia coli
0.195
-
ATP pH 7.2, 37°C, mutant enzyme G216A Escherichia coli
0.196
-
L-lysine pH 7.2, 37°C, mutant enzyme G216A Escherichia coli
0.203
-
L-lysine pH 7.2, 37°C, mutant enzyme F426H Escherichia coli
0.254
-
L-lysine pH 7.2, 37°C, mutant enzyme E278D Escherichia coli
0.83
-
ATP pH 7.2, 37°C, mutant enzyme N424Q Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-lysine + tRNALys
-
Escherichia coli AMP + L-lysyl-tRNALys + diphosphate
-
?

Synonyms

Synonyms Comment Organism
class II lysyl-tRNA synthetase
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0087
-
L-lysine pH 7.2, 37°C, mutant enzyme E240Q Escherichia coli
0.013
-
ATP pH 7.2, 37°C, mutant enzyme E240Q Escherichia coli
0.015
-
L-lysine pH 7.2, 37°C, mutant enzyme E278Q Escherichia coli
0.017
-
ATP pH 7.2, 37°C, mutant enzyme E278Q Escherichia coli
0.017
-
ATP pH 7.2, 37°C, mutant enzyme E428Q Escherichia coli
0.02
-
L-lysine pH 7.2, 37°C, mutant enzyme E428D Escherichia coli
0.031
-
L-lysine pH 7.2, 37°C, mutant enzyme Y280F Escherichia coli
0.054
-
ATP pH 7.2, 37°C, mutant enzyme E278D Escherichia coli
0.086
-
L-lysine pH 7.2, 37°C, mutant enzyme E240D Escherichia coli
0.14
-
ATP pH 7.2, 37°C, mutant enzyme E240D Escherichia coli
0.16
-
L-lysine pH 7.2, 37°C, mutant enzyme E278D Escherichia coli
0.17
-
ATP pH 7.2, 37°C, mutant enzyme Y280F Escherichia coli
0.35
-
ATP pH 7.2, 37°C, mutant enzyme Y280S Escherichia coli
0.6
-
L-lysine pH 7.2, 37°C, mutant enzyme F426W Escherichia coli
0.65
-
L-lysine pH 7.2, 37°C, mutant enzyme N424Q Escherichia coli
0.67
-
ATP pH 7.2, 37°C, mutant enzyme F426H Escherichia coli
0.8
-
ATP pH 7.2, 37°C, mutant enzyme G216A Escherichia coli
1
-
ATP pH 7.2, 37°C, mutant enzyme E428D Escherichia coli
1
-
L-lysine pH 7.2, 37°C, mutant enzyme E428D Escherichia coli
1
-
ATP pH 7.2, 37°C, mutant enzyme N424D Escherichia coli
1.6
-
L-lysine pH 7.2, 37°C, mutant enzyme N424D Escherichia coli
1.6
-
L-lysine pH 7.2, 37°C, mutant enzyme Y280S Escherichia coli
1.8
-
L-lysine pH 7.2, 37°C, wild-type enzyme Escherichia coli
2.2
-
ATP pH 7.2, 37°C, mutant enzyme N424Q Escherichia coli
2.4
-
L-lysine pH 7.2, 37°C, mutant enzyme F426H Escherichia coli
2.4
-
ATP pH 7.2, 37°C, mutant enzyme F426W Escherichia coli
3.4
-
ATP pH 7.2, 37°C, wild-type enzyme Escherichia coli
3.4
-
L-lysine pH 7.2, 37°C, mutant enzyme G216A Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00001
-
5'-O-[N-(L-lysyl)sulfamoyl]adenosine pH 7.2, 37°C, mutant enzyme E240Q Escherichia coli
0.000011
-
5'-O-[N-(L-lysyl)sulfamoyl]adenosine pH 7.2, 37°C, mutant enzyme E428Q Escherichia coli
0.000023
-
5'-O-[N-(L-lysyl)sulfamoyl]adenosine pH 7.2, 37°C, mutant enzyme E278Q Escherichia coli
0.000028
-
5'-O-[N-(L-lysyl)sulfamoyl]adenosine pH 7.2, 37°C, wild-type enzyme Escherichia coli
0.000029
-
5'-O-[N-(L-lysyl)sulfamoyl]adenosine pH 7.2, 37°C, mutant enzyme E240D Escherichia coli
0.000039
-
5'-O-[N-(L-lysyl)sulfamoyl]adenosine pH 7.2, 37°C, mutant enzyme N424D Escherichia coli
0.000041
-
5'-O-[N-(L-lysyl)sulfamoyl]adenosine pH 7.2, 37°C, mutant enzyme E278D Escherichia coli
0.00005
-
5'-O-[N-(L-lysyl)sulfamoyl]adenosine pH 7.2, 37°C, mutant enzyme F426W Escherichia coli
0.000055
-
5'-O-[N-(L-lysyl)sulfamoyl]adenosine pH 7.2, 37°C, mutant enzyme N424Q Escherichia coli
0.000059
-
5'-O-[N-(L-lysyl)sulfamoyl]adenosine pH 7.2, 37°C, mutant enzyme G216A Escherichia coli
0.000061
-
5'-O-[N-(L-lysyl)sulfamoyl]adenosine pH 7.2, 37°C, mutant enzyme E428D Escherichia coli
0.00022
-
5'-O-[N-(L-lysyl)sulfamoyl]adenosine pH 7.2, 37°C, mutant enzyme Y280S Escherichia coli
0.00027
-
5'-O-[N-(L-lysyl)sulfamoyl]adenosine pH 7.2, 37°C, mutant enzyme Y280F Escherichia coli
0.0013
-
5'-O-[N-(L-lysyl)sulfamoyl]adenosine pH 7.2, 37°C, mutant enzyme F426H Escherichia coli
0.0015
-
S-(2-aminoethyl)-L-cysteine pH 7.2, 37°C, mutant enzyme E428Q Escherichia coli
0.0031
-
S-(2-aminoethyl)-L-cysteine pH 7.2, 37°C, mutant enzyme E240D Escherichia coli
0.0039
-
S-(2-aminoethyl)-L-cysteine pH 7.2, 37°C, wild-type enzyme Escherichia coli
0.004
-
S-(2-aminoethyl)-L-cysteine pH 7.2, 37°C, mutant enzyme N424D Escherichia coli
0.005
-
S-(2-aminoethyl)-L-cysteine pH 7.2, 37°C, mutant enzyme E278Q Escherichia coli
0.0054
-
S-(2-aminoethyl)-L-cysteine pH 7.2, 37°C, mutant enzyme E240Q Escherichia coli
0.025
-
S-(2-aminoethyl)-L-cysteine pH 7.2, 37°C, mutant enzyme F426W Escherichia coli
0.034
-
S-(2-aminoethyl)-L-cysteine pH 7.2, 37°C, mutant enzyme E428D Escherichia coli
0.072
-
S-(2-aminoethyl)-L-cysteine pH 7.2, 37°C, mutant enzyme Y280F Escherichia coli
0.076
-
S-(2-aminoethyl)-L-cysteine pH 7.2, 37°C, mutant enzyme N424Q Escherichia coli
0.34
-
S-(2-aminoethyl)-L-cysteine pH 7.2, 37°C, mutant enzyme Y280S Escherichia coli
1.24
-
S-(2-aminoethyl)-L-cysteine pH 7.2, 37°C, mutant enzyme G216A Escherichia coli
1.29
-
S-(2-aminoethyl)-L-cysteine pH 7.2, 37°C, mutant enzyme E278D Escherichia coli
1.31
-
S-(2-aminoethyl)-L-cysteine pH 7.2, 37°C, mutant enzyme F426H Escherichia coli