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Literature summary for 6.1.1.6 extracted from

  • Ibba, M.; Losey, H.C.; Kawarabayasi, Y.; Kikuchi, H.; Bunjun, S.; Soll, D.
    Substrate recognition by class I lysyl-tRNA synthetases: a molecular basis for gene displacement (1999), Proc. Natl. Acad. Sci. USA, 96, 418-423.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.6

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination Aeropyrum pernix
expression in strain BL21(DE3) as His-tagged protein Escherichia coli
gene lysS, constitutive expression in Escherichia coli DH5alpha, functional complementation of a class II-enzyme-deficient Escherichia coli mutant only with addition of exogenous lysine Methanococcus maripaludis
gene lysS, constitutive expression in Escherichia coli DH5alpha, functional complementation of a class II-enzyme-deficient Escherichia coli mutant up to 40°C growth temperature with or without addition of exogenous lysine Borreliella burgdorferi

Inhibitors

Inhibitors Comment Organism Structure
CNBr treatment of tRNALys substrate with CNBr reduces the enzyme activity Methanococcus maripaludis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
 Escherichia coli wild type tRNA recombinant enzyme, pH 7.2, 37°C Borreliella burgdorferi
0.0019
-
 Escherichia coli wild type tRNA recombinant enzyme, pH 7.2, 37°C Escherichia coli
0.002
-
 Borellia burgdorferi tRNALys recombinant enzyme, pH 7.2, 37°C Borreliella burgdorferi
0.0039
-
 Escherichia coli G2.U71 tRNA recombinant enzyme, pH 7.2, 37°C Borreliella burgdorferi
0.0043
-
 Borellia burgdorferi tRNALys recombinant enzyme, pH 7.2, 37°C Escherichia coli
0.0063
-
 Escherichia coli G2.U71 tRNA recombinant enzyme, pH 7.2, 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-lysine + tRNALys Escherichia coli
-
 AMP + L-lysyl-tRNALys + diphosphate
-
 ?
ATP + L-lysine + tRNALys Methanococcus maripaludis
-
 AMP + L-lysyl-tRNALys + diphosphate
-
 ?
ATP + L-lysine + tRNALys Borreliella burgdorferi
-
 AMP + L-lysyl-tRNALys + diphosphate
-
 ?
ATP + L-lysine + tRNALys Aeropyrum pernix
-
 AMP + L-lysyl-tRNALys + diphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix Q9YFT9 class I enzyme
-
Borreliella burgdorferi
-
 class I enzyme, purified recombinant enzyme expressed in Escherichia coli
-
Escherichia coli
-
 class II enzyme
-
Methanococcus maripaludis
-
 class I enzyme, purified recombinant enzyme expressed in Escherichia coli DH5alpha
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged protein from stran BL21(DE3), functional complementation of a class II-enzyme-deficient Escherichia coli mutant Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-lysine + Borellia burgdorferi tRNALys
-
 Escherichia coli AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + Borellia burgdorferi tRNALys
-
 Borreliella burgdorferi AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + Escherichia coli G2.U71 tRNA
-
 Escherichia coli ?
-
 ?
ATP + L-lysine + Escherichia coli G2.U71 tRNA
-
 Borreliella burgdorferi ?
-
 ?
ATP + L-lysine + Escherichia coli G2.U71 tRNALys
-
 Escherichia coli AMP + diphosphate + L-lysyl-tRNALys
-
 r
ATP + L-lysine + Escherichia coli G2.U71 tRNALys
-
 Borreliella burgdorferi AMP + diphosphate + L-lysyl-tRNALys
-
 r
ATP + L-lysine + Escherichia coli tRNALys
-
 Methanococcus maripaludis AMP + diphosphate + L-lysyl-tRNALys
-
 r
ATP + L-lysine + Escherichia coli tRNALys CNBr-treated
-
 Methanococcus maripaludis AMP + diphosphate + L-lysyl-tRNALys CNBr-treated
-
 r
ATP + L-lysine + Escherichia coli wild type tRNA
-
 Escherichia coli ?
-
 ?
ATP + L-lysine + Escherichia coli wild type tRNA
-
 Borreliella burgdorferi ?
-
 ?
ATP + L-lysine + Escherichia coli wild type tRNALys
-
 Escherichia coli AMP + diphosphate + L-lysyl-tRNALys
-
 r
ATP + L-lysine + Escherichia coli wild type tRNALys
-
 Borreliella burgdorferi AMP + diphosphate + L-lysyl-tRNALys
-
 r
ATP + L-lysine + Methanococcus maripaludis tRNALys
-
 Methanococcus maripaludis AMP + diphosphate + L-lysyl-tRNALys
-
 r
ATP + L-lysine + Methanococcus maripaludis tRNALys CNBr-treated
-
 Methanococcus maripaludis AMP + diphosphate + L-lysyl-tRNALys CNBr-treated
-
 r
ATP + L-lysine + tRNALys
-
 Escherichia coli AMP + L-lysyl-tRNALys + diphosphate
-
 ?
ATP + L-lysine + tRNALys
-
 Methanococcus maripaludis AMP + L-lysyl-tRNALys + diphosphate
-
 ?
ATP + L-lysine + tRNALys
-
 Borreliella burgdorferi AMP + L-lysyl-tRNALys + diphosphate
-
 ?
ATP + L-lysine + tRNALys
-
 Aeropyrum pernix AMP + L-lysyl-tRNALys + diphosphate
-
 r
ATP + L-lysine + tRNALys predicted secondary structure of the tRNALys from Methanococcus maripaludis Methanococcus maripaludis AMP + L-lysyl-tRNALys + diphosphate
-
 ?
ATP + L-lysine + tRNALys substrate recognition mechanism Aeropyrum pernix AMP + L-lysyl-tRNALys + diphosphate
-
 r
ATP + L-lysine + tRNALys tRNA substrate from Borrelia burgdorferi or Escherichia coli, predicted secondary structure of the tRNALys from Borrelia burgdorferi Borreliella burgdorferi AMP + L-lysyl-tRNALys + diphosphate
-
 ?
ATP + L-lysine + tRNALys tRNA substrate from Borrelia burgdorferi or Escherichia coli, predicted secondary structure of tRNAlys from Escherichia coli Escherichia coli AMP + L-lysyl-tRNALys + diphosphate
-
 ?
additional information substrate recognition specificity, the enzyme also performs the ATP-diphosphate exchange reaction, class I lysine-tRNA synthetases recognize the same elements in tRNALys as their class II counterparts, namely the discriminator base N73 and the anticodon, but vary in the recognition of the G2.U71 wobble pair of spirochete tRNALys, which acts as a determinant for class II enzymes, but not for class I enzymes Borreliella burgdorferi ?
-
 ?
additional information substrate recognition specificity, the enzyme also performs the ATP-diphosphate exchange reaction, class II lysine-tRNA synthetases recognize the same elements in tRNALys as their class I counterparts, namely the discriminator base N73 and the anticodon, but vary in the recognition of the G2.U71 wobble pair of spirochete tRNALys, which acts as a determinant for class II enzymes, but not for class I enzymes Escherichia coli ?
-
 ?
additional information the enzyme also performs the ATP-diphosphate exchange reaction, class I lysine-tRNA synthetases recognize the same elements in tRNALys as their class II counterparts, namely the discriminator base N73 and the anticodon, but vary in the recognition of the G2.U71 wobble pair of spirochete tRNALys, which acts as a determinant for class II enzymes, but not for class I enzymes Methanococcus maripaludis ?
-
 ?
additional information the enzyme also performs the ATP-diphosphate exchange reaction, class I lysine-tRNA synthetases recognize the same elements in tRNALys as their class II counterparts, namely the discriminator base N73 and the anticodon, but vary in the recognition of the G2.U71 wobble pair of spirochete tRNALys, which acts as a determinant for class II enzymes, but not for class I enzymes Aeropyrum pernix ?
-
 ?

Synonyms

Synonyms Comment Organism
L-Lysine-transfer RNA ligase
-
 Escherichia coli
L-Lysine-transfer RNA ligase
-
 Methanococcus maripaludis
L-Lysine-transfer RNA ligase
-
 Borreliella burgdorferi
L-Lysine-transfer RNA ligase
-
 Aeropyrum pernix
Lysine translase
-
 Escherichia coli
Lysine translase
-
 Methanococcus maripaludis
Lysine translase
-
 Borreliella burgdorferi
Lysine translase
-
 Aeropyrum pernix
Lysine--tRNA ligase
-
 Escherichia coli
Lysine--tRNA ligase
-
 Methanococcus maripaludis
Lysine--tRNA ligase
-
 Borreliella burgdorferi
Lysine--tRNA ligase
-
 Aeropyrum pernix
Lysine-tRNA synthetase
-
 Escherichia coli
Lysine-tRNA synthetase
-
 Methanococcus maripaludis
Lysine-tRNA synthetase
-
 Borreliella burgdorferi
Lysine-tRNA synthetase
-
 Aeropyrum pernix
LysRS
-
 Escherichia coli
LysRS
-
 Methanococcus maripaludis
LysRS
-
 Borreliella burgdorferi
LysRS
-
 Aeropyrum pernix
Lysyl-transfer ribonucleate synthetase
-
 Escherichia coli
Lysyl-transfer ribonucleate synthetase
-
 Methanococcus maripaludis
Lysyl-transfer ribonucleate synthetase
-
 Borreliella burgdorferi
Lysyl-transfer ribonucleate synthetase
-
 Aeropyrum pernix
Lysyl-transfer RNA synthetase
-
 Escherichia coli
Lysyl-transfer RNA synthetase
-
 Methanococcus maripaludis
Lysyl-transfer RNA synthetase
-
 Borreliella burgdorferi
Lysyl-transfer RNA synthetase
-
 Aeropyrum pernix
Lysyl-tRNA synthetase
-
 Escherichia coli
Lysyl-tRNA synthetase
-
 Methanococcus maripaludis
Lysyl-tRNA synthetase
-
 Borreliella burgdorferi
Lysyl-tRNA synthetase
-
 Aeropyrum pernix
Synthetase, lysyl-transfer ribonucleate
-
 Escherichia coli
Synthetase, lysyl-transfer ribonucleate
-
 Methanococcus maripaludis
Synthetase, lysyl-transfer ribonucleate
-
 Borreliella burgdorferi
Synthetase, lysyl-transfer ribonucleate
-
 Aeropyrum pernix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli
37
-
 assay at Methanococcus maripaludis
37
-
 assay at Borreliella burgdorferi
37
-
 assay at Aeropyrum pernix

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 Escherichia coli tRNALys CNBr-treated recombinant enzyme, pH 7.2, 37°C Methanococcus maripaludis
0.0025
-
 Escherichia coli G2.U71 tRNA recombinant enzyme, pH 7.2, 37°C Escherichia coli
0.0035
-
 Borellia burgdorferi tRNALys recombinant enzyme, pH 7.2, 37°C Escherichia coli
0.0035
-
 Escherichia coli G2.U71 tRNA recombinant enzyme, pH 7.2, 37°C Borreliella burgdorferi
0.004
-
 Escherichia coli tRNALys recombinant enzyme, pH 7.2, 37°C Methanococcus maripaludis
0.004
-
 Escherichia coli wild type tRNALys recombinant enzyme, pH 7.2, 37°C Borreliella burgdorferi
0.05
-
 Methanococcus maripaludis tRNALys CNBr-treated recombinant enzyme, pH 7.2, 37°C Methanococcus maripaludis
0.055
-
 Escherichia coli wild type tRNALys recombinant enzyme, pH 7.2, 37°C Escherichia coli
0.2
-
 Methanococcus maripaludis tRNALys recombinant enzyme, pH 7.2, 37°C Methanococcus maripaludis
0.79
-
 Borellia burgdorferi tRNALys recombinant enzyme, pH 7.2, 37°C Borreliella burgdorferi
6.08
-
 Borellia burgdorferi tRNALys recombinant enzyme, pH 7.2, 37°C Borreliella burgdorferi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Escherichia coli
7.2
-
 assay at Methanococcus maripaludis
7.2
-
 assay at Borreliella burgdorferi
7.2
-
 assay at Aeropyrum pernix

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli
ATP
-
 Methanococcus maripaludis
ATP
-
 Borreliella burgdorferi
ATP
-
 Aeropyrum pernix