Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme alone or in complex with L-lysine, 0.001 ml of protein solution 5 mg/ml, with or without 10 mM L-lysine, with an equal volume of reservoir solution: 120 mM HEPES, pH 7.5, 2.4 M ammonium sulfate, 2.4% v/v PEG 400, 20°C, X-ray diffraction structure determination at 2.6 A for the enzyme crystals and at 2.9 A for the complex crystals, structure analysis | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | Pyrococcus horikoshii | - |
AMP + L-lysyl-tRNALys + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O57963 | class I enzyme, i.e. LysRS-I | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys | mechanism of tRNALys and L-lysine binding | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | - |
Pyrococcus horikoshii | AMP + L-lysyl-tRNALys + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-Lysine-transfer RNA ligase | - |
Pyrococcus horikoshii |
Lysine translase | - |
Pyrococcus horikoshii |
Lysine--tRNA ligase | - |
Pyrococcus horikoshii |
Lysine-tRNA synthetase | - |
Pyrococcus horikoshii |
LysRS | - |
Pyrococcus horikoshii |
LysRS-I | - |
Pyrococcus horikoshii |
LysRS-II | - |
Pyrococcus horikoshii |
Lysyl-transfer ribonucleate synthetase | - |
Pyrococcus horikoshii |
Lysyl-transfer RNA synthetase | - |
Pyrococcus horikoshii |
Lysyl-tRNA synthetase | - |
Pyrococcus horikoshii |
More | enzyme has structural similarity to glutamyl-tRNA synthetase, EC 6.1.1.17, from other organisms, e.g. Thermus thermophilus | Pyrococcus horikoshii |
Synthetase, lysyl-transfer ribonucleate | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pyrococcus horikoshii |