Any feedback?
Literature summary for 6.1.1.6 extracted from
- The N-terminal domain of mammalian Lysyl-tRNA synthetase is a functional tRNA-binding domain (2002), J. Biol. Chem., 277, 1762-1769.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the C-terminally His-tagged N-terminal extension of the enzyme in Escherichia coli strain BL21(DE3) | Cricetinae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | binding kinetics of acceptor stem and anticodon domains of the tRNALys | Cricetinae | |
| 0.002 | - |
yeast tRNALys | recombinant wild-type enzyme, pH 7.5, 25°C | Cricetinae | |
| 0.0028 | - |
yeast tRNALys | recombinant N-terminally truncated enzyme, pH 7.5, 25°C | Cricetinae | |
| 0.003 | - |
human tRNALys3 | recombinant wild-type enzyme, pH 7.5, 25°C | Cricetinae | |
| 0.0117 | - |
human tRNALys3 | recombinant N-terminally truncated enzyme, pH 7.5, 25°C | Cricetinae | |
| 0.092 | - |
L-lysine | recombinant wild-type enzyme, pH 7.5, 25°C | Cricetinae |  |
| 0.104 | - |
L-lysine | recombinant N-terminally truncated enzyme, pH 7.5, 25°C | Cricetinae | |
| 0.195 | - |
ATP | recombinant N-terminally truncated enzyme, pH 7.5, 25°C | Cricetinae | |
| 0.25 | - |
ATP | recombinant wild-type enzyme, pH 7.5, 25°C | Cricetinae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 8608 | - |
recombinant N-terminal extension, mass spectrometry | Cricetinae |
| 8741 | - |
recombinant N-terminal extension, DNA sequence determination | Cricetinae |
| 9104 | - |
recombinant N-terminal extension, centrifugation sedimentation equilibrium | Cricetinae |
| 24700 | - |
recombinant N-terminal extension, gel filtration | Cricetinae |
| 62000 | - |
2 * 62000, N-terminally truncated recombinant enzyme, SDS-PAGE | Cricetinae |
| 120000 | - |
N-terminally truncated recombinant enzyme, gel filtration | Cricetinae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + lysine + tRNALys | Cricetinae | - |
AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cricetinae | - |
purified recombinant enzyme expressed in Saccharomyces cerevisiae, wild-type and N-terminally truncated form, the latter prepared by proteolytic cleavage by elastase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His-tagged N-terminal extension of the enzyme from expression in Escherichia coli, to homogeneity | Cricetinae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-lysine + human tRNALys3 | - |
Cricetinae | AMP + diphosphate + L-lysyl-tRNALys3 | - |
? | |
| ATP + L-lysine + yeast tRNALys | - |
Cricetinae | AMP + diphosphate + L-lysyl-tRNALys | - |
? | |
| ATP + lysine + tRNALys | - |
Cricetinae | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
| ATP + lysine + tRNALys | tRNALys from Saccharomyces cerevisiae, recombinant N-terminal extension provides the enzyme with RNA binding properties, the N-terminally truncated enzyme is no longer able to bind the RNA substrate | Cricetinae | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 62000, N-terminally truncated recombinant enzyme, SDS-PAGE | Cricetinae |
| More | recombinant N-terminal extension is a monomer in solution with an elongated shape | Cricetinae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-Lysine-transfer RNA ligase | - |
Cricetinae |
| Lysine translase | - |
Cricetinae |
| Lysine--tRNA ligase | - |
Cricetinae |
| Lysine-tRNA synthetase | - |
Cricetinae |
| LysRS | - |
Cricetinae |
| Lysyl-transfer ribonucleate synthetase | - |
Cricetinae |
| Lysyl-transfer RNA synthetase | - |
Cricetinae |
| Lysyl-tRNA synthetase | - |
Cricetinae |
| Synthetase, lysyl-transfer ribonucleate | - |
Cricetinae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Cricetinae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5 | - |
ATP | recombinant wild-type enzyme, pH 7.5, 25°C | Cricetinae | |
| 5.1 | - |
ATP | recombinant N-terminally truncated enzyme, pH 7.5, 25°C | Cricetinae | |
| 5.1 | - |
L-lysine | recombinant N-terminally truncated enzyme, pH 7.5, 25°C | Cricetinae | |
| 5.8 | - |
L-lysine | recombinant wild-type enzyme, pH 7.5, 25°C | Cricetinae | |
| 6.2 | - |
human tRNALys3 | recombinant wild-type enzyme, pH 7.5, 25°C | Cricetinae | |
| 6.3 | - |
yeast tRNALys | recombinant wild-type enzyme, pH 7.5, 25°C | Cricetinae | |
| 7.1 | - |
yeast tRNALys | recombinant N-terminally truncated enzyme, pH 7.5, 25°C | Cricetinae | |
| 7.8 | - |
human tRNALys3 | recombinant N-terminally truncated enzyme, pH 7.5, 25°C | Cricetinae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Cricetinae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Cricetinae | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Cricetinae | recombinant N-terminal extension, calculation | - |
8.1 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
