Literature summary for 6.1.1.6 extracted from

Onesti, S.; Desogus, G.; Brevet, A.; Chen, J.; Plateau, P.; Blanquet, S.; Brick, P.
Structural studies of lysyl-tRNA synthetase: conformational changes induced by substrate binding (2000), Biochemistry, 39, 12853-12861.

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
unliganded enzyme and in complex with L-lysine, vapor diffusion hanging drop method, protein solution: 20 mg/ml protein, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol, 10 mM MgCl2, plus equal volume of reservoir solution: 0.1 M HEPES, pH 7.5, 46-50% saturated ammonium sulfate solution, 2-4% polyethylene glycol 400, 15-20% glycerol, 4-18°C, X-ray difraction structure determination at 2.7 A resolution, and analysis	Escherichia coli

Crystallization (Comment)

Organism

unliganded enzyme and in complex with L-lysine, vapor diffusion hanging drop method, protein solution: 20 mg/ml protein, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol, 10 mM MgCl2, plus equal volume of reservoir solution: 0.1 M HEPES, pH 7.5, 46-50% saturated ammonium sulfate solution, 2-4% polyethylene glycol 400, 15-20% glycerol, 4-18°C, X-ray difraction structure determination at 2.7 A resolution, and analysis

Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58000	-	2 * 58000, recombinant enzyme, crystal structure	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-lysine + tRNALys	Escherichia coli	-	AMP + L-lysyl-tRNALys + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A8N3	class II enzyme	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Escherichia coli

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys	active site structure and substrate binding	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-lysine + tRNALys	-	Escherichia coli	AMP + L-lysyl-tRNALys + diphosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 58000, recombinant enzyme, crystal structure	Escherichia coli

Synonyms

Synonyms	Comment	Organism
L-Lysine-transfer RNA ligase	-	Escherichia coli
Lysine translase	-	Escherichia coli
Lysine--tRNA ligase	-	Escherichia coli
Lysine-tRNA synthetase	-	Escherichia coli
LysRS	-	Escherichia coli
Lysyl-transfer ribonucleate synthetase	-	Escherichia coli
Lysyl-transfer RNA synthetase	-	Escherichia coli
Lysyl-tRNA synthetase	-	Escherichia coli
Synthetase, lysyl-transfer ribonucleate	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli