Literature summary for 6.1.1.5 extracted from

Istvan, E.S.; Dharia, N.V.; Bopp, S.E.; Gluzman, I.; Winzeler, E.A.; Goldberg, D.E.
Validation of isoleucine utilization targets in Plasmodium falciparum (2011), Proc. Natl. Acad. Sci. USA, 108, 1627-1632.

Search for more literature for 6.1.1.5

Application

Application	Comment	Organism
drug development	isoleucine utilization is an essential pathway that can be targeted for antimalarial drug development	Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment)	Organism
genotyping of thiaisoleucine-resistant enzymes, expression of GFP-tagged cytoplasmic isozyme in erythrocytic stage parasite	Plasmodium falciparum

Protein Variants

Protein Variants	Comment	Organism
L810F	naturally occuring mutation in the cytoplasmic IleRS responsible for thiaisoleucine-resistance in the parasite, phenotype, overview	Plasmodium falciparum

Inhibitors

Inhibitors	Comment	Organism	Structure
mupirocin	irreversible inhibition, inhibits development of invasion-competent parasites in the second asexual cycle, delayed death phenotype	Plasmodium falciparum
thiaisoleucine	directly competes with isoleucine for a target, irreversible inhibition, inhibits ring-stage parasites in development	Plasmodium falciparum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
apicoplast	-	Plasmodium falciparum	20011	-
cytoplasm	-	Plasmodium falciparum	5737	-
additional information	two IRS isozymes localize to distinct compartments in the parasite	Plasmodium falciparum	-	-

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	-	two isozymes, an apicoplast isozyme encoded by gene PFL1210w, and a cytoplasmic isozyme encoded by gene PF13_0179	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	the cytoplasmic isozyme gene is expressed during the erythrocytic stages and is localized in the proper compartment for the bulk of protein synthesis, while the apicoplast isozyme is expressed at low rates in the erythrocytic stage and gets more abundant in later developmental stages, overview	Plasmodium falciparum	-

Synonyms

Synonyms	Comment	Organism
Isoleucyl-tRNA synthetase	-	Plasmodium falciparum

General Information

General Information	Comment	Organism
additional information	thiaisoleucine-resistant parasites possess a mutation in the cytoplasmic isoleucyl-tRNA synthetase, mutational analysis, overview	Plasmodium falciparum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)