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Literature summary for 6.1.1.5 extracted from

  • Salowe, S.P.; Wiltsie, J.; Hawkins, J.C.; Sonatore, L.M.
    The catalytic flexibility of tRNAIle-lysidine synthetase can generate alternative tRNA substrates for isoleucyl-tRNA synthetase (2009), J. Biol. Chem., 284, 9656-9662.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.5

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET3D-FLAG for expression in Escherichia coli BL21DE3 cells Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-isoleucine + tRNAIle Bacillus subtilis bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine, the lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by IleRS AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?
ATP + L-isoleucine + tRNAIle Bacillus subtilis 168 bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine, the lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by IleRS AMP + diphosphate + L-isoleucyl-tRNAIle
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 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis Q45477
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-
Bacillus subtilis 168 Q45477
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-

Purification (Commentary)

Purification (Comment) Organism
using M2-FLAG resin Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-isoleucine + tRNAIle bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine, the lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by IleRS Bacillus subtilis AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?
ATP + L-isoleucine + tRNAIle bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine, the lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by IleRS Bacillus subtilis 168 AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?

Synonyms

Synonyms Comment Organism
IleRS
-
 Bacillus subtilis
Isoleucyl-tRNA synthetase
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 activity assay at room temperature Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 activity assay Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Bacillus subtilis