Crystallization (Comment) | Organism |
---|---|
crystallization of the 5'-N-[N-(L-isoleucyl)sulfamoyl]adenosine-enzyme complex, and the muciproin-enzyme complex by preparation of enzyme crystals and soaking of the crystals in 0.1 mM 5'-N-[N-(L-isoleucyl)sulfamoyl]adenosine solution, and 1 mM muciproin solution, respectively, X-ray diffraction structure determination at 2.5 A resolution, and analysis | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
H581L/L583H | site-directed mutagenesis, slightly reduced enzyme activity | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-N-[N-(L-isoleucyl)sulfamoyl]adenosine | non-hydrolyzable analogue of the reaction intermediate Ile-AMP | Thermus thermophilus | |
muciproin | - |
Staphylococcus aureus | |
muciproin | inhibition by blockage of the binding site of high energy intermediate Ile-AMP, the inhibitor contains a moiety that morphologically resembles the hydrophobic side chain of L-isoleucine, recognition is mediated by Pro46, Trp518, and Trp558 | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.118 | - |
L-isoleucine | mutant enzyme | Staphylococcus aureus | |
52.8 | - |
L-isoleucine | pH 8.0, 65°C, recombinant wild-type enzyme | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNAIle | Thermus thermophilus | - |
AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
ATP + L-isoleucine + tRNAIle | Staphylococcus aureus | - |
AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
ATP + L-isoleucine + tRNAIle | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | P41368 | - |
- |
Thermus thermophilus | - |
purified recombinant wild-type and mutant enzyme | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
purified recombinant wild-type and mutant enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-isoleucine + tRNAIle = AMP + diphosphate + L-isoleucyl-tRNAIle | substrate recognition mechanism, Glu551, Thr48, His581 and Lys594are involved | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNAIle | - |
Thermus thermophilus | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
ATP + L-isoleucine + tRNAIle | - |
Staphylococcus aureus | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
ATP + L-isoleucine + tRNAIle | enzyme shows a common recognition mode of aminoacyl-adenylate for a class I aminoacyl-tRNA synthetase, formation of high-energy reaction intermediate Ile-AMP | Thermus thermophilus | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
ATP + L-isoleucine + tRNAIle | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
ATP + L-isoleucine + tRNAIle | enzyme shows a common recognition mode of aminoacyl-adenylate for a class I aminoacyl-tRNA synthetase, formation of high-energy reaction intermediate Ile-AMP | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IleRS | - |
Thermus thermophilus |
IleRS | - |
Staphylococcus aureus |
IRS | - |
Thermus thermophilus |
IRS | - |
Staphylococcus aureus |
Isoleucine translase | - |
Thermus thermophilus |
Isoleucine translase | - |
Staphylococcus aureus |
Isoleucine--tRNA ligase | - |
Thermus thermophilus |
Isoleucine--tRNA ligase | - |
Staphylococcus aureus |
Isoleucine-transfer RNA ligase | - |
Thermus thermophilus |
Isoleucine-transfer RNA ligase | - |
Staphylococcus aureus |
Isoleucine-tRNA synthetase | - |
Thermus thermophilus |
Isoleucine-tRNA synthetase | - |
Staphylococcus aureus |
Isoleucyl-transfer ribonucleate synthetase | - |
Thermus thermophilus |
Isoleucyl-transfer ribonucleate synthetase | - |
Staphylococcus aureus |
Isoleucyl-transfer RNA synthetase | - |
Thermus thermophilus |
Isoleucyl-transfer RNA synthetase | - |
Staphylococcus aureus |
Isoleucyl-tRNA synthetase | - |
Thermus thermophilus |
Isoleucyl-tRNA synthetase | - |
Staphylococcus aureus |
Mupirocin resistance protein | - |
Thermus thermophilus |
Mupirocin resistance protein | - |
Staphylococcus aureus |
Synthetase, isoleucyl-transfer ribonucleate | - |
Thermus thermophilus |
Synthetase, isoleucyl-transfer ribonucleate | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermus thermophilus | |
ATP | - |
Staphylococcus aureus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Thermus thermophilus | |
0.00025 | - |
muciproin | pH 8.0, 65°C, recombinant wild-type enzyme, with respect to L-isoleucine | Thermus thermophilus | |
0.0023 | - |
muciproin | mutant enzyme | Staphylococcus aureus |