Activating Compound | Comment | Organism | Structure |
---|---|---|---|
spermidine | in presence of 50 mM K+, addition of spermine prevents inhibitory effect of 1.5 mM Mg2+ or above. In presence of 200 mM K+, the addition of spermine stimulates isoleucyl-tRNA formation in presence of Mg2+, 0-5 mM | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | in presence of 50 mM K+ and in absence of polyamines, the optimal Mg2+ concentration for Ile-tRNA formation is 1 mM, an increase in Mg2+ concentration markedly inhibits | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | in presence of 50 mM K+ and in absence of polyamines, the optimal Mg2+ concentration for Ile-tRNA formation is 1 mM, an increase in Mg2+ concentration markedly inhibits | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00084 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNAIle | - |
Rattus norvegicus | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? |