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Literature summary for 6.1.1.4 extracted from

  • Zhai, Y.; Martinis, S.A.
    Two conserved threonines collaborate in the Escherichia coli leucyl-tRNA synthetase amino acid editing mechanism (2005), Biochemistry, 44, 15437-15443.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
T247A/T248A 533fold decrease in the ratio of turnover number to Km-value compared to wild-type ratio Escherichia coli
T247S/T248S 77fold decrease in the ratio of turnover number to Km-value compared to wild-type ratio Escherichia coli
T247V 8fold decrease in the ratio of turnover number to Km-value compared to wild-type ratio Escherichia coli
T247V/T248V fold decrease in the ratio of turnover number to Km-value compared to wild-type ratio Escherichia coli
T248V 6fold decrease in the ratio of turnover number to Km-value compared to wild-type ratio Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
tRNALeu(UAA) pH 7.5, 37°C, wild-type enzyme Escherichia coli
0.0022
-
tRNALeu(UAA) pH 7.5, 37°C, mutant enzyme T248V Escherichia coli
0.0024
-
tRNALeu(UAA) pH 7.5, 37°C, mutant enzyme T247S/T248S Escherichia coli
0.0026
-
tRNALeu(UAA) pH 7.5, 37°C, mutant enzyme T247V Escherichia coli
0.0036
-
tRNALeu(UAA) pH 7.5, 37°C, mutant enzyme T247A/T248A Escherichia coli
0.0044
-
tRNALeu(UAA) pH 7.5, 37°C, mutant enzyme T247V/T248V Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu(UAA)
-
Escherichia coli AMP + diphosphate + L-leucyl-tRNALeu(UAA)
-
?
additional information Thr247 and Thr248 are two key residues in the Escherichia coli LeuRS editing active site and appear to collaborate in the hydrolytic cleavage mechanism Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
LeuRS
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
tRNALeu(UAA) pH 7.5, 37°C, mutant enzyme T247V/T248V Escherichia coli
0.1
-
tRNALeu(UAA) pH 7.5, 37°C, mutant enzyme T247A/T248A Escherichia coli
0.8
-
tRNALeu(UAA) pH 7.5, 37°C, mutant enzyme T247V Escherichia coli
0.9
-
tRNALeu(UAA) pH 7.5, 37°C, mutant enzyme T248V Escherichia coli
3.2
-
tRNALeu(UAA) pH 7.5, 37°C, wild-type enzyme Escherichia coli
5.2
-
tRNALeu(UAA) pH 7.5, 37°C, mutant enzyme T247S/T248S Escherichia coli