Protein Variants | Comment | Organism |
---|---|---|
T247A/T248A | 533fold decrease in the ratio of turnover number to Km-value compared to wild-type ratio | Escherichia coli |
T247S/T248S | 77fold decrease in the ratio of turnover number to Km-value compared to wild-type ratio | Escherichia coli |
T247V | 8fold decrease in the ratio of turnover number to Km-value compared to wild-type ratio | Escherichia coli |
T247V/T248V | fold decrease in the ratio of turnover number to Km-value compared to wild-type ratio | Escherichia coli |
T248V | 6fold decrease in the ratio of turnover number to Km-value compared to wild-type ratio | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
tRNALeu(UAA) | pH 7.5, 37°C, wild-type enzyme | Escherichia coli | |
0.0022 | - |
tRNALeu(UAA) | pH 7.5, 37°C, mutant enzyme T248V | Escherichia coli | |
0.0024 | - |
tRNALeu(UAA) | pH 7.5, 37°C, mutant enzyme T247S/T248S | Escherichia coli | |
0.0026 | - |
tRNALeu(UAA) | pH 7.5, 37°C, mutant enzyme T247V | Escherichia coli | |
0.0036 | - |
tRNALeu(UAA) | pH 7.5, 37°C, mutant enzyme T247A/T248A | Escherichia coli | |
0.0044 | - |
tRNALeu(UAA) | pH 7.5, 37°C, mutant enzyme T247V/T248V | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu(UAA) | - |
Escherichia coli | AMP + diphosphate + L-leucyl-tRNALeu(UAA) | - |
? | |
additional information | Thr247 and Thr248 are two key residues in the Escherichia coli LeuRS editing active site and appear to collaborate in the hydrolytic cleavage mechanism | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LeuRS | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
tRNALeu(UAA) | pH 7.5, 37°C, mutant enzyme T247V/T248V | Escherichia coli | |
0.1 | - |
tRNALeu(UAA) | pH 7.5, 37°C, mutant enzyme T247A/T248A | Escherichia coli | |
0.8 | - |
tRNALeu(UAA) | pH 7.5, 37°C, mutant enzyme T247V | Escherichia coli | |
0.9 | - |
tRNALeu(UAA) | pH 7.5, 37°C, mutant enzyme T248V | Escherichia coli | |
3.2 | - |
tRNALeu(UAA) | pH 7.5, 37°C, wild-type enzyme | Escherichia coli | |
5.2 | - |
tRNALeu(UAA) | pH 7.5, 37°C, mutant enzyme T247S/T248S | Escherichia coli |