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Literature summary for 6.1.1.4 extracted from

  • Mursinna, R.S.; Lee, K.W.; Briggs, J.M.; Martinis, S.A.
    Molecular dissection of a critical specificity determinant within the amino acid editing domain of leucyl-tRNA synthetase (2004), Biochemistry, 43, 155-165.
    View publication on PubMed
Search for more literature for 6.1.1.4

Protein Variants

Protein Variants Comment Organism
T252A the ratio of turnover-number to KM-value for L-leucine in aminoacylation is 5% of the wild-type ratio, the ratio of turnover-number to KM-value for tRNALeu in aminoacylation is 10% of the wild-type ratio. The ratio of turnover-number to Km value for Leu-tRNALeu(UAA) is 25fold higher than the wild-type value, the ratio of turnover-number to Km value for Ile-tRNALeu(UAA) is 1.25fold higher than the wild-type value Escherichia coli
T252S the ratio of turnover-number to KM-value for L-leucine in aminoacylation is 60% of the wild-type ratio, the ratio of turnover-number to KM-value for tRNALeu in aminoacylation is 80% of the wild-type ratio. The ratio of turnover-number to Km value for Leu-tRNALeu(UAA) is 7.5fold higher than the wild-type value, the ratio of turnover-number to Km value for Ile-tRNALeu(UAA) is 1.6fold lower than the wild-type value Escherichia coli
T252V the ratio of turnover-number to KM-value for L-leucine in aminoacylation is identical to wild-type ratio, the ratio of turnover-number to KM-value for tRNALeu in aminoacylation is 90% of the wild-type ratio. The ratio of turnover-number to Km value for Leu-tRNALeu(UAA) is 1.75fold higher than the wild-type value, the ratio of turnover-number to Km value for Ile-tRNALeu(UAA) is 13.3fold higher than the wild-type value Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 tRNALeu(UAA) wild-type enzyme, aminoacylation Escherichia coli
0.0015
-
 tRNALeu(UAA) mutant enzyme T252V, aminoacylation Escherichia coli
0.0015
-
 L-leucine wild-type enzyme, aminoacylation Escherichia coli
0.0016
-
 L-leucine mutant enzyme T252V, aminoacylation Escherichia coli
0.0017
-
 tRNALeu(UAA) mutant enzyme T252S, aminoacylation Escherichia coli
0.002
-
 L-leucine mutant enzyme T252S, aminoacylation Escherichia coli
0.0028
-
 tRNALeu(UAA) mutant enzyme T252A, aminoacylation Escherichia coli
0.0036
-
 L-leucine mutant enzyme T252A, aminoacylation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu(UAA)
-
 Escherichia coli AMP + diphosphate + L-leucyl-tRNALeu(UAA)
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 L-leucine mutant enzyme T252A, aminoacylation Escherichia coli
1.6
-
 tRNALeu(UAA) mutant enzyme T252A, aminoacylation Escherichia coli
5.1
-
 L-leucine mutant enzyme T252S, aminoacylation Escherichia coli
6.1
-
 L-leucine wild-type enzyme, aminoacylation Escherichia coli
6.1
-
 tRNALeu(UAA) wild-type enzyme, aminoacylation Escherichia coli
6.2
-
 L-leucine mutant enzyme T252V, aminoacylation Escherichia coli
6.3
-
 tRNALeu(UAA) mutant enzyme T252V, aminoacylation Escherichia coli
6.5
-
 tRNALeu(UAA) mutant enzyme T252S, aminoacylation Escherichia coli