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Literature summary for 6.1.1.4 extracted from
- Human mitochondrial leucyl-tRNA synthetase with high activity produced from Escherichia coli (2003), Protein Expr. Purif., 30, 112-116.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the mitochondrial isozyme is cloned and expressed in Escherichia coli with the same N-terminus as that processed in the mitochondria of insect cells as His-tagged protein | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
L-leucine | recombinant mitochondrial isozyme mutant, 37°C | Homo sapiens |  |
| 0.021 | - |
L-leucine | recombinant mitochondrial isozyme mutant, 37°C | Homo sapiens | |
| 0.99 | - |
ATP | recombinant mitochondrial isozyme mutant, 37°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-leucine + tRNALeu | Homo sapiens | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | processing of the mitochondrial isozyme by removing of the mitochondrial targeting presequence in the mitochondria of insect cells | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally mutated His-tagged mitochondrial isozyme | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.011 | - |
recombinant mitochondrial isozyme mutant in cell lysate | Homo sapiens |
| 0.128 | - |
purified recombinant mitochondrial isozyme mutant | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-leucine + tRNALeu | - |
Homo sapiens | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
| additional information | activity with mitochondrial tRNA mutants associated with some human mitochondrion-related neuromuscular disorders | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Leucine translase | - |
Homo sapiens |
| Leucine--tRNA ligase | - |
Homo sapiens |
| Leucyl-transfer ribonucleate synthetase | - |
Homo sapiens |
| Leucyl-transfer ribonucleic acid synthetase | - |
Homo sapiens |
| Leucyl-transfer RNA synthetase | - |
Homo sapiens |
| Leucyl-tRNA synthetase | - |
Homo sapiens |
| LeuRS | - |
Homo sapiens |
| Synthetase, leucyl-transfer ribonucleate | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
L-leucine | recombinant mitochondrial isozyme mutant, 37°C | Homo sapiens | |
| 0.22 | - |
ATP | recombinant mitochondrial isozyme mutant, 37°C | Homo sapiens | |
| 0.23 | - |
L-leucine | recombinant mitochondrial isozyme mutant, 37°C | Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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