Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.4 extracted from

  • Xu, M.G.; Chen, J.F.; Martin, F.; Zhao, M.W.; Eriani, G.; Wang, E.D.
    Leucyl-tRNA synthetase consisting of two subunits from hyperthermophilic bacteria Aquifex aeolicus (2002), J. Biol. Chem., 277, 41590-41596.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of the enzyme and the beta-subunit alone in Escherichia coli, the alpha-subunit cannot be expressed stably Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
tRNALeu from Aquifex aeolicus aminoacylation reaction, pH 7.8, 60°C Aquifex aeolicus
0.00038
-
tRNALeu from Aquifex aeolicus aminoacylation reaction, pH 7.8, 37°C Aquifex aeolicus
0.00076
-
tRNALeu from Escherichia coli aminoacylation reaction, pH 7.8, 37°C Aquifex aeolicus
0.0013
-
L-leucine ATP-diphosphate exchange reaction, pH 7.8, 37°C Aquifex aeolicus
0.0015
-
tRNALeu from Escherichia coli aminoacylation reaction, pH 7.8, 60°C Aquifex aeolicus
0.0016
-
L-leucine ATP-diphosphate exchange reaction, pH 7.8, 60°C Aquifex aeolicus
0.006
-
L-leucine aminoacylation reaction, pH 7.8, 60°C Aquifex aeolicus
0.0064
-
L-leucine aminoacylation reaction, pH 7.8, 37°C Aquifex aeolicus
0.11
-
ATP aminoacylation reaction, pH 7.8, 60°C Aquifex aeolicus
0.36
-
ATP ATP-diphosphate exchange reaction, pH 7.8, 37°C Aquifex aeolicus
0.38
-
ATP ATP-diphosphate exchange reaction, pH 7.8, 60°C Aquifex aeolicus
0.55
-
ATP aminoacylation reaction, pH 7.8, 37°C Aquifex aeolicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal concentration is 12 mM Aquifex aeolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33500
-
recombinant beta-subunit, gel filtration Aquifex aeolicus
74000
-
1 * 74000, alpha-subunit, + 1 * 33500, beta-subunit, SDS-PAGE Aquifex aeolicus
107500
-
recombinant enzyme, gel filtration Aquifex aeolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-leucine + tRNALeu Aquifex aeolicus
-
AMP + diphosphate + L-leucyl-tRNALeu
-
?

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme and beta-subunit from Escherichia coli, to near homogeneity Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu
-
Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
?
ATP + L-leucine + tRNALeu tRNALeu from Aquifex aeolicus and Escherichia coli, native and recombinant wild-type, the recombinant isolated beta-subunit is inactive in catalysis Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
?
ATP + L-leucine + tRNALeu from Aquifex aeolicus
-
Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
?
ATP + L-leucine + tRNALeu from Escherichia coli
-
Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
?
additional information the enzyme also performs the ATP-diphosphate exchange reaction Aquifex aeolicus ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 74000, alpha-subunit, + 1 * 33500, beta-subunit, SDS-PAGE Aquifex aeolicus
More determination od secondary structure elements in the enzyme and the isolated beta-subunit Aquifex aeolicus

Synonyms

Synonyms Comment Organism
Leucine translase
-
Aquifex aeolicus
Leucine--tRNA ligase
-
Aquifex aeolicus
Leucyl-transfer ribonucleate synthetase
-
Aquifex aeolicus
Leucyl-transfer ribonucleic acid synthetase
-
Aquifex aeolicus
Leucyl-transfer RNA synthetase
-
Aquifex aeolicus
Leucyl-tRNA synthetase
-
Aquifex aeolicus
LeuRS
-
Aquifex aeolicus
Synthetase, leucyl-transfer ribonucleate
-
Aquifex aeolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Aquifex aeolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
stable up to under optimal conditions Aquifex aeolicus
75
-
30 min, stable Aquifex aeolicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.084
-
tRNALeu from Escherichia coli aminoacylation reaction, pH 7.8, 37°C Aquifex aeolicus
0.39
-
L-leucine aminoacylation reaction, pH 7.8, 37°C Aquifex aeolicus
0.39
-
tRNALeu from Aquifex aeolicus aminoacylation reaction, pH 7.8, 37°C Aquifex aeolicus
0.4
-
tRNALeu from Escherichia coli aminoacylation reaction, pH 7.8, 60°C Aquifex aeolicus
1.4
-
L-leucine aminoacylation reaction, pH 7.8, 60°C Aquifex aeolicus
1.5
-
tRNALeu from Aquifex aeolicus aminoacylation reaction, pH 7.8, 60°C Aquifex aeolicus
1.8
-
ATP aminoacylation reaction, pH 7.8, 60°C Aquifex aeolicus
3.3
-
ATP ATP-diphosphate exchange reaction, pH 7.8, 37°C Aquifex aeolicus
3.5
-
L-leucine ATP-diphosphate exchange reaction, pH 7.8, 37°C Aquifex aeolicus
3.9
-
ATP aminoacylation reaction, pH 7.8, 37°C Aquifex aeolicus
14.5
-
ATP ATP-diphosphate exchange reaction, pH 7.8, 60°C Aquifex aeolicus
15.5
-
L-leucine ATP-diphosphate exchange reaction, pH 7.8, 60°C Aquifex aeolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Aquifex aeolicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Aquifex aeolicus