Cloned (Comment) | Organism |
---|---|
expression of the enzyme and the beta-subunit alone in Escherichia coli, the alpha-subunit cannot be expressed stably | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
tRNALeu from Aquifex aeolicus | aminoacylation reaction, pH 7.8, 60°C | Aquifex aeolicus | |
0.00038 | - |
tRNALeu from Aquifex aeolicus | aminoacylation reaction, pH 7.8, 37°C | Aquifex aeolicus | |
0.00076 | - |
tRNALeu from Escherichia coli | aminoacylation reaction, pH 7.8, 37°C | Aquifex aeolicus | |
0.0013 | - |
L-leucine | ATP-diphosphate exchange reaction, pH 7.8, 37°C | Aquifex aeolicus | |
0.0015 | - |
tRNALeu from Escherichia coli | aminoacylation reaction, pH 7.8, 60°C | Aquifex aeolicus | |
0.0016 | - |
L-leucine | ATP-diphosphate exchange reaction, pH 7.8, 60°C | Aquifex aeolicus | |
0.006 | - |
L-leucine | aminoacylation reaction, pH 7.8, 60°C | Aquifex aeolicus | |
0.0064 | - |
L-leucine | aminoacylation reaction, pH 7.8, 37°C | Aquifex aeolicus | |
0.11 | - |
ATP | aminoacylation reaction, pH 7.8, 60°C | Aquifex aeolicus | |
0.36 | - |
ATP | ATP-diphosphate exchange reaction, pH 7.8, 37°C | Aquifex aeolicus | |
0.38 | - |
ATP | ATP-diphosphate exchange reaction, pH 7.8, 60°C | Aquifex aeolicus | |
0.55 | - |
ATP | aminoacylation reaction, pH 7.8, 37°C | Aquifex aeolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal concentration is 12 mM | Aquifex aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33500 | - |
recombinant beta-subunit, gel filtration | Aquifex aeolicus |
74000 | - |
1 * 74000, alpha-subunit, + 1 * 33500, beta-subunit, SDS-PAGE | Aquifex aeolicus |
107500 | - |
recombinant enzyme, gel filtration | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | Aquifex aeolicus | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme and beta-subunit from Escherichia coli, to near homogeneity | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | - |
Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | tRNALeu from Aquifex aeolicus and Escherichia coli, native and recombinant wild-type, the recombinant isolated beta-subunit is inactive in catalysis | Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu from Aquifex aeolicus | - |
Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu from Escherichia coli | - |
Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
additional information | the enzyme also performs the ATP-diphosphate exchange reaction | Aquifex aeolicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 74000, alpha-subunit, + 1 * 33500, beta-subunit, SDS-PAGE | Aquifex aeolicus |
More | determination od secondary structure elements in the enzyme and the isolated beta-subunit | Aquifex aeolicus |
Synonyms | Comment | Organism |
---|---|---|
Leucine translase | - |
Aquifex aeolicus |
Leucine--tRNA ligase | - |
Aquifex aeolicus |
Leucyl-transfer ribonucleate synthetase | - |
Aquifex aeolicus |
Leucyl-transfer ribonucleic acid synthetase | - |
Aquifex aeolicus |
Leucyl-transfer RNA synthetase | - |
Aquifex aeolicus |
Leucyl-tRNA synthetase | - |
Aquifex aeolicus |
LeuRS | - |
Aquifex aeolicus |
Synthetase, leucyl-transfer ribonucleate | - |
Aquifex aeolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Aquifex aeolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
stable up to under optimal conditions | Aquifex aeolicus |
75 | - |
30 min, stable | Aquifex aeolicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.084 | - |
tRNALeu from Escherichia coli | aminoacylation reaction, pH 7.8, 37°C | Aquifex aeolicus | |
0.39 | - |
L-leucine | aminoacylation reaction, pH 7.8, 37°C | Aquifex aeolicus | |
0.39 | - |
tRNALeu from Aquifex aeolicus | aminoacylation reaction, pH 7.8, 37°C | Aquifex aeolicus | |
0.4 | - |
tRNALeu from Escherichia coli | aminoacylation reaction, pH 7.8, 60°C | Aquifex aeolicus | |
1.4 | - |
L-leucine | aminoacylation reaction, pH 7.8, 60°C | Aquifex aeolicus | |
1.5 | - |
tRNALeu from Aquifex aeolicus | aminoacylation reaction, pH 7.8, 60°C | Aquifex aeolicus | |
1.8 | - |
ATP | aminoacylation reaction, pH 7.8, 60°C | Aquifex aeolicus | |
3.3 | - |
ATP | ATP-diphosphate exchange reaction, pH 7.8, 37°C | Aquifex aeolicus | |
3.5 | - |
L-leucine | ATP-diphosphate exchange reaction, pH 7.8, 37°C | Aquifex aeolicus | |
3.9 | - |
ATP | aminoacylation reaction, pH 7.8, 37°C | Aquifex aeolicus | |
14.5 | - |
ATP | ATP-diphosphate exchange reaction, pH 7.8, 60°C | Aquifex aeolicus | |
15.5 | - |
L-leucine | ATP-diphosphate exchange reaction, pH 7.8, 60°C | Aquifex aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Aquifex aeolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Aquifex aeolicus |