Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.4 extracted from

  • Gouda, M.; Yokogawa, T.; Asahara, H.; Nishikawa, K.
    Leucyl-tRNA synthetase from the extreme thermophile Aquifex aeolicus has a heterodimeric quaternary structure (2002), FEBS Lett., 518, 139-143.
    View publication on PubMed
Search for more literature for 6.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
2 genes leuS and leuS', overexpression in Escherichia coli BL21(DE3) Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 tRNALeu from Escherichia coli recombinant enzyme complex, 55°C Aquifex aeolicus
0.0014
-
 tRNALeu from Aquifex aeolicus recombinant enzyme complex, 55°C Aquifex aeolicus
0.0083
-
 L-leucine recombinant enzyme complex, 65°C Aquifex aeolicus
0.228
-
 ATP recombinant enzyme complex, 65°C Aquifex aeolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 heterodimer in quartenary structure, 2 * 35000, leuS gene product, + 2 * 65000, leuS gene product, SDS-PAGE Aquifex aeolicus
65000
-
 heterodimer in quartenary structure, 2 * 35000, leuS gene product, + 2 * 65000, leuS gene product, SDS-PAGE Aquifex aeolicus
100000
-
 complexed recombinant leuS' and leuS gene product, gel filtration Aquifex aeolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-leucine + tRNALeu Aquifex aeolicus
-
 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
 class I enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzymes from Escherichia coli, leuS' gene product to homogeneity Aquifex aeolicus

Renatured (Commentary)

Renatured (Comment) Organism
renaturation and refolding of recombinant leuS gene product expressed in Escherichia coli Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu
-
 Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
ATP + L-leucine + tRNALeu enzyme activity only appears when both gene products, of leuS and leuS', coexist Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
ATP + L-leucine + tRNALeu from Aquifex aeolicus
-
 Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
ATP + L-leucine + tRNALeu from Escherichia coli
-
 Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Subunits

Subunits Comment Organism
tetramer heterodimer in quartenary structure, 2 * 35000, leuS' gene product, + 2 * 65000, leuS gene product, SDS-PAGE Aquifex aeolicus

Synonyms

Synonyms Comment Organism
Leucine translase
-
 Aquifex aeolicus
Leucine--tRNA ligase
-
 Aquifex aeolicus
Leucyl-transfer ribonucleate synthetase
-
 Aquifex aeolicus
Leucyl-transfer ribonucleic acid synthetase
-
 Aquifex aeolicus
Leucyl-transfer RNA synthetase
-
 Aquifex aeolicus
Leucyl-tRNA synthetase
-
 Aquifex aeolicus
LeuRS
-
 Aquifex aeolicus
Synthetase, leucyl-transfer ribonucleate
-
 Aquifex aeolicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
 tRNALeu from Escherichia coli recombinant enzyme complex, 55°C Aquifex aeolicus
0.006
-
 tRNALeu from Aquifex aeolicus recombinant enzyme complex, 55°C Aquifex aeolicus
0.094
-
 ATP recombinant enzyme complex, 65°C Aquifex aeolicus
0.134
-
 L-leucine recombinant enzyme complex, 65°C Aquifex aeolicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Aquifex aeolicus