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Literature summary for 6.1.1.4 extracted from
- Phaseolus vulgaris cytoplasmic leucyl-tRNA synthetase. Purification and comparison of its catalytic, structural, and immunological properties with those of the chloroplastic enzyme (1983), J. Biol. Chem., 258, 12386-12393.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Phaseolus vulgaris | 9507 | - |
| cytoplasm | - |
Phaseolus vulgaris | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimal Mg2+/ATP ratio: 1.5 | Phaseolus vulgaris |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
1 * 120000, chloroplastic enzyme, SDS-PAGE of denatured enzyme | Phaseolus vulgaris |
| 130000 | - |
1 * 130000, cytoplasmic enzyme, SDS-PAGE of denatured enzyme | Phaseolus vulgaris |
| 135000 | - |
gel filtration | Phaseolus vulgaris |
| 135000 | - |
chloroplastic enzyme | Phaseolus vulgaris |
| 200000 | - |
cytoplasmic enzyme, gel filtration | Phaseolus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.94 | - |
- |
Phaseolus vulgaris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol, stable for several weeks | Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-leucine + tRNALeu | cytoplasmic enzyme shows less strict specificity towards tRNA than the chloroplastic enzyme | Phaseolus vulgaris | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 120000, chloroplastic enzyme, SDS-PAGE of denatured enzyme | Phaseolus vulgaris |
| monomer | 1 * 130000, cytoplasmic enzyme, SDS-PAGE of denatured enzyme | Phaseolus vulgaris |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.05 | - |
Leu-tRNALeu | - |
Phaseolus vulgaris | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
cytoplasmic enzyme | Phaseolus vulgaris |
| 9 | - |
chloroplastic enzyme | Phaseolus vulgaris |
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Release 2023.1 (January 2023)
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