General Stability | Organism |
---|---|
20-30% loss of activity after 1 cycle of freezing and thawing | Euglena gracilis |
stability is lower with decreasing protein concentration | Euglena gracilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Euglena gracilis | 9507 | - |
cytoplasm | - |
Euglena gracilis | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
1 * 100000, chloroplastic enzyme, SDS-PAGE | Euglena gracilis |
116000 | - |
1 * 116000, cytoplasmic enzyme, SDS-PAGE | Euglena gracilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Euglena gracilis |
Storage Stability | Organism |
---|---|
4°C, 50 mM potassium phosphate buffer, pH 7.0, 1 mM MgCl2, 0.002 mM L-leucine, 100 mM NaCl, 15% v/v 1,2-propanediol, stable for several weeks | Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | - |
Euglena gracilis | AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 116000, cytoplasmic enzyme, SDS-PAGE | Euglena gracilis |
monomer | 1 * 100000, chloroplastic enzyme, SDS-PAGE | Euglena gracilis |