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Literature summary for 6.1.1.4 extracted from

  • Chiu, A.O.S.; Suyama, Y.
    The absence of structural relationship between mitochondrial and cytoplasmic leucyl-tRNA synthetases from Tetrahymena pyriformis (1975), Arch. Biochem. Biophys., 171, 43-54.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Tetrahymena pyriformis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Tetrahymena pyriformis 5737
-
mitochondrion
-
Tetrahymena pyriformis 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 5.0 mM, mitochondrial enzyme, 2.5 mM, cytoplasmic enzyme Tetrahymena pyriformis
Mg2+ optimal concentration: 2.5 mM (mitochondrial enzyme), 10 mM (cytoplasmic enzyme) Tetrahymena pyriformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000 100000 gel filtration, sucrose density gradient centrifugation Tetrahymena pyriformis
100000
-
1 * 100000, SDS-PAGE Tetrahymena pyriformis

Organism

Organism UniProt Comment Textmining
Tetrahymena pyriformis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Tetrahymena pyriformis

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, stable for at least 3 months Tetrahymena pyriformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu
-
Tetrahymena pyriformis AMP + diphosphate + L-leucyl-tRNALeu
-
?

Subunits

Subunits Comment Organism
monomer 1 * 100000, SDS-PAGE Tetrahymena pyriformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
mitochondrial enzyme Tetrahymena pyriformis
35
-
cytoplasmic enzyme Tetrahymena pyriformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 40 5°C: about 35% of maximal activity, 40°C: about 45% of maximal activity, mitochondrial enzyme Tetrahymena pyriformis
20 40 20°C: about 30% of maximal activity, 40°C: about 85% of maximal activity, cytoplasmic enzyme Tetrahymena pyriformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
2.5 min, 90% loss of activity of mitochondrial enzyme, 5-10 min, 90% loss of cytoplasmic enzyme Tetrahymena pyriformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 mitochondrial enzyme Tetrahymena pyriformis
7 8 cytoplasmic enzyme Tetrahymena pyriformis