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Literature summary for 6.1.1.4 extracted from
- Purification and properties of leucyl-tRNA synthetase from bakers' yeast (1973), J. Biol. Chem., 248, 1074-1079.
General Stability
| General Stability | Organism |
|---|---|
| DTT stabilizes | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | in presence of 0.1 mM Mg2+ | Saccharomyces cerevisiae |  |
| DTNB | - |
Saccharomyces cerevisiae | |
| DTT | - |
Saccharomyces cerevisiae | |
| p-chloromercuribenzoate | - |
Saccharomyces cerevisiae | |
| Zn2+ | in presence of 0.1 mM Mg2+ | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
tRNALeu | - |
Saccharomyces cerevisiae | |
| 0.02 | - |
L-Leu | - |
Saccharomyces cerevisiae | |
| 0.11 | - |
ATP | - |
Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | Mg2+, Mn2+, Ca2+ and Ba2+ similarly effective, optimal concentration: 0.1 mM | Saccharomyces cerevisiae | |
| Ca2+ | Mg2+, Mn2+, Ca2+ and Ba2+ similarly effective, optimal concentration: 0.1 mM | Saccharomyces cerevisiae | |
| Mg2+ | Mg2+, Mn2+, Ca2+ and Ba2+ similarly effective, optimal concentration: 0.1 mM | Saccharomyces cerevisiae | |
| Mn2+ | Mg2+, Mn2+, Ca2+ and Ba2+ similarly effective, optimal concentration: 0.1 mM | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 119000 | 120000 | equilibrium sedimentation method | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.804 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -90°C | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-leucine + tRNALeu | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
| additional information | no substrates: dATP, GTP, dGTP | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55-60000, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 33 | 37 | - |
Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
rapid inactivation | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
in cacodylate(K+) buffer or HEPES buffer | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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