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Literature summary for 6.1.1.3 extracted from

  • Ye, W.; Spanning, E.; Glaser, E.; Maeler, L.
    Interaction of the dual targeting peptide of Thr-tRNA synthetase with the chloroplastic receptor Toc34 in Arabidopsis thaliana (2015), FEBS open bio, 5, 405-412 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.3

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
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 Arabidopsis thaliana 9507
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additional information in Arabidopsis thaliana, 18 aminoacyl-tRNA synthetases, aaRSs, including of AtThr-tRNA synthetase, are dually targeted to mitochondria and chloroplasts. The C-terminal unstructured AtThrRS-dTP(30-60) part of the dual targeting peptide reveals very strong inhibition (90%) of the chloroplast import and affects the mitochondrial import to much lower extent (25%). The mitochondrial receptor Tom20 interacts mostly with the N-terminal portion of the AtThrRS-dTP(2-60) peptide, whereas the chloroplastic receptor Toc34 interacts with both the N- and C-terminal segments of the dual targeting peptide. The interaction of Toc34 with the C-terminal part of AtThrRS-dTP(2-60) shows that a helical structure is not required Arabidopsis thaliana
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-threonine + tRNAThr Arabidopsis thaliana
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 AMP + diphosphate + L-threonyl-tRNAThr
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 ?
additional information Arabidopsis thaliana interaction of the dual targeting peptide of Thr-tRNA synthetase with the chloroplastic receptor Toc34 in Arabidopsis thaliana as a function of AtToc34 concentration. Mapping the AtToc34 interaction sites in AtThrRS-dTP(2-60), overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana F4IFC5
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-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-threonine + tRNAThr
-
 Arabidopsis thaliana AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
additional information interaction of the dual targeting peptide of Thr-tRNA synthetase with the chloroplastic receptor Toc34 in Arabidopsis thaliana as a function of AtToc34 concentration. Mapping the AtToc34 interaction sites in AtThrRS-dTP(2-60), overview Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
Thr-tRNA synthetase
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 Arabidopsis thaliana
ThrRS
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 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism in Arabidopsis thaliana, 18 aminoacyl-tRNA synthetases, aaRSs, including of AtThr-tRNA synthetase, are dually targeted to mitochondria and chloroplasts Arabidopsis thaliana
physiological function interaction of the dual targeting peptide of Thr-tRNA synthetase with the chloroplastic receptor Toc34 (AtToc34) in Arabidopsis thaliana, analysis of mode of interactions of a dual targeting peptide with both Tom20 and Toc34. Effect of peptides corresponding to different segments of AtThrRS-dTP on in vitro import of organelle specific proteins. The N-terminal A2-Y29 segment of AtThrRS-dTP is essential for import into both organelles, while the C-terminal L30-P60 part is important for chloroplastic import efficiency. The recognition of the dual targeting peptide of AtThr-tRNA synthetase is different for the mitochondrial and chloroplastic receptors Arabidopsis thaliana