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Literature summary for 6.1.1.3 extracted from

  • Ling, J.; Soell, D.
    Severe oxidative stress induces protein mistranslation through impairment of an aminoacyl-tRNA synthetase editing site (2010), Proc. Natl. Acad. Sci. USA, 107, 4028-4033.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
hydrogen peroxide oxidizes cysteine182 residue critical for editing, which leads to Ser-tRNAThr formation and protein mistranslation that impaired growth of Escherichia coli. Presence of major heat shock proteases is required to allow cell growth in medium containing serine and hydrogen peroxide, which suggests that the mistranslated proteins are misfolded Escherichia coli
additional information reactive oxygen species cause editing defect and misacylation by WT ThrRS Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of diphosphate exchange activities and threonylation of tRNAThr of ThrRS with or without H2O2 treatment, overview Escherichia coli
0.0015
-
tRNAThr pH 7.2, 37°C, ThrRS, aminoacylation with Thr Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-threonine + tRNAThr Escherichia coli
-
AMP + diphosphate + L-threonyl-tRNAThr
-
?
ATP + L-threonine + tRNAThr Escherichia coli MG1655
-
AMP + diphosphate + L-threonyl-tRNAThr
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli MG1655
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-threonine + tRNAThr
-
Escherichia coli AMP + diphosphate + L-threonyl-tRNAThr
-
?
ATP + L-threonine + tRNAThr
-
Escherichia coli MG1655 AMP + diphosphate + L-threonyl-tRNAThr
-
?

Synonyms

Synonyms Comment Organism
Threonyl-tRNA synthetase
-
Escherichia coli
ThrRS
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
tRNAThr pH 7.2, 37°C, ThrRS, aminoacylation with Thr Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli

General Information

General Information Comment Organism
malfunction reactive oxygen species cause editing defect and misacylation by WT ThrRS. H2O2-induced Ser-tRNAThr formation causes protein mistranslation Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
38.3
-
tRNAThr pH 7.2, 37°C, ThrRS, aminoacylation with Thr Escherichia coli