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Literature summary for 6.1.1.3 extracted from

  • Berglund, A.K.; Spanning, E.; Biverstahl, H.; Maddalo, G.; Tellgren-Roth, C.; Maeler, L.; Glaser, E.
    Dual targeting to mitochondria and chloroplasts: Characterization of Thr-tRNA synthetase targeting peptide (2009), Mol. Plant, 2, 1298-1309.
    View publication on PubMed
Search for more literature for 6.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
deletion constructs of the ThrRS-dual targeting peptide, ThrRS-dTP, are generated, consisting of amino acid residues 1-50, 1-60, or 24-68, ligated into the pCR Blunt II TOPO vector as a fusion protein with GFP located C-terminally, for a GST-ThrRS-dTP(1-60) construct the vector pGEX-6P-2 is used Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-
mitochondrion
-
 Arabidopsis thaliana 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6600
-
 ThrRS-dual targeting peptide, determined by SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-threonine + tRNAThr Arabidopsis thaliana
-
 AMP + diphosphate + L-threonyl-tRNAThr
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a RESOURCE S column Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-threonine + tRNAThr
-
 Arabidopsis thaliana AMP + diphosphate + L-threonyl-tRNAThr
-
 ?

Synonyms

Synonyms Comment Organism
Thr-tRNA synthetase
-
 Arabidopsis thaliana
ThrRS
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function the final localisation of Thr-tRNA synthetase, expressed as a precursor protein, depends on the dual targeting peptide ThrRS-dTP Arabidopsis thaliana