Cloned (Comment) | Organism |
---|---|
overexpression in strain IBPC6881 as His-tagged enzyme | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
W434Y | reduced activity, Trp434 is involved in conformational changes during substrate binding | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and kinetic mechanism | Escherichia coli | |
0.201 | - |
L-threonine | wild-type enzyme, pH 7.2, 37°C | Escherichia coli | |
0.267 | - |
ATP | wild-type enzyme, pH 7.2, 37°C | Escherichia coli | |
0.387 | - |
ATP | mutant enzyme W434Y, pH 7.2, 37°C | Escherichia coli | |
0.897 | - |
L-threonine | mutant enzyme W434Y, pH 7.2, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-threonine + tRNAThr | Escherichia coli | - |
AMP + diphosphate + L-threonyl-tRNAThr | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from strain IBPC6881, to homogeneity | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr | induced fit, Trp434 is involved in conformational changes during substrate binding | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-threonine + tRNAThr | - |
Escherichia coli | AMP + diphosphate + L-threonyl-tRNAThr | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Synthetase, threonyl-transfer ribonucleate | - |
Escherichia coli |
Threonine translase | - |
Escherichia coli |
Threonine--tRNA ligase | - |
Escherichia coli |
Threonine-transfer ribonucleate synthetase | - |
Escherichia coli |
Threonyl-ribonucleic synthetase | - |
Escherichia coli |
Threonyl-transfer ribonucleate synthetase | - |
Escherichia coli |
Threonyl-transfer ribonucleic acid synthetase | - |
Escherichia coli |
Threonyl-transfer RNA synthetase | - |
Escherichia coli |
Threonyl-tRNA synthetase | - |
Escherichia coli |
ThrRS | - |
Escherichia coli |
TRS | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
L-threonine | mutant enzyme W434Y, pH 7.2, 37°C | Escherichia coli | |
42 | - |
ATP | mutant enzyme W434Y, pH 7.2, 37°C | Escherichia coli | |
90 | - |
ATP | wild-type enzyme, pH 7.2, 37°C | Escherichia coli | |
90 | - |
L-threonine | wild-type enzyme, pH 7.2, 37°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |